Horie Yoshifumi, Takahashi Chiho
Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Nakano Shimoshinjo, Akita, 010-0195, Japan.
Research Center for Inland Sea (KURCIS), Kobe University, Fukae Minami Kobe, Hyogo, 658-0022, Higashinada, Japan.
Environ Monit Assess. 2021 Oct 14;193(11):725. doi: 10.1007/s10661-021-09527-8.
To determine whether the marine medaka Oryzias melastigma is a suitable model organism for in vivo acute toxicity bioassay in seawater, we first determined whether there were differences in the concentrations of chemicals that were toxic to marine medaka (O. melastigma) and freshwater medaka (O. latipes). We performed in vivo acute toxicity bioassay with 3-chloroaniline, triclosan, 3,4-dichloroaniline, fenitrothion, and pyriproxyfen on larvae of both species. Although the concentrations of 3-chloroaniline and fenitrothion that were lethal to the larvae were identical for both species, the toxic concentrations of triclosan, 3,4-dichloroaniline, and pyriproxyfen were lower for O. melastigma than for O. latipes. We then used an in vivo acute toxicity bioassay to monitor the quality of coastal seawater in Akita, Japan. No lethal effects were observed in the harbor and canal in 2019. O. melastigma could be used to monitor the quality of seawater with salinities in the range 2-25. Our findings suggest that O. melastigma can be used as the test fish for in vivo acute toxicity bioassay intended for water quality monitoring.
为了确定日本青鳉（Oryzias melastigma）是否是用于海水中体内急性毒性生物测定的合适模式生物，我们首先确定了对日本青鳉（O. melastigma）和青鳉（O. latipes）有毒的化学物质浓度是否存在差异。我们对这两个物种的幼体进行了3-氯苯胺、三氯生、3,4-二氯苯胺、杀螟硫磷和吡丙醚的体内急性毒性生物测定。虽然对两个物种的幼体致死的3-氯苯胺和杀螟硫磷浓度相同，但三氯生、3,4-二氯苯胺和吡丙醚对日本青鳉的毒性浓度低于青鳉。然后，我们使用体内急性毒性生物测定来监测日本秋田沿海海水的质量。2019年在港口和运河未观察到致死效应。日本青鳉可用于监测盐度范围为2-25的海水质量。我们的研究结果表明，日本青鳉可作为用于水质监测的体内急性毒性生物测定的试验鱼。
