[ study on the effects of intercellular adhesion operon of on the inflammation associated with bacteria-fungal mixed biofilm].

OBJECTIVE

To study the effect of intercellular adhesion (ica) operon of on the inflammation associated with mixed biofilm of and on endotracheal tube material in rabbits.

METHODS

The standard strains of RP62A (ica operon positive, positive group) and ATCC12228 (ica operon negative, negative group) were taken to prepare a bacterial solution with a concentration of 1×10 CFU/mL, respectively. Then, the two bacterial solutions were mixed with the standard strain of ATCC10231 of the same concentration to prepare a mixed culture solution at a ratio of 1∶1, respectively. The mixed culture solution was incubated with endotracheal tube material for 24 hours. The formation of mixed biofilm on the surface of the material was observed by scanning electron microscope. Thirty New Zealand rabbits, aged 4-6 months, were divided into two groups ( =15), and the endotracheal tube materials of the positive group and the negative group that were incubated for 24 hours were implanted beside the trachea. The body mass of rabbits in the two groups was measured before operation and at 1, 3, and 7 days after operation. At 1, 3, and 7 days after operation, the levels of interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), and monocytechemotactic protein 1 (MCP-1) were detected by using an ELISA test kit. At 7 days after operation, the formation of mixed biofilm on the surface of the endotracheal tube materials was observed by scanning electron microscope, the inflammation and infiltration of tissues around the materials were observed by HE staining, and the bacterial infections in heart, lung, liver, and kidney were observed by plate colony counting method.

RESULTS

Scanning electron microscope observation showed that the mixed biofilm structure was obvious in the positive group after 24 hours incubation, but no mixed biofilm formation was observed in the negative group. studies showed that there was no significant difference in body mass between the two groups before operation and at 1, 3, and 7 days after operation ( >0.05). Compared with the negative group, the levels of MCP-1 and IL-1β at 1 day, and the levels of IL-1β, MCP-1, IL-6, and TNF-α at 3 and 7 days in the positive group all increased, with significant differences ( <0.05). Scanning electron microscope observation showed that a large amount of and mixed biofilm structure were observed in the positive group, and a very small amount of bacteria was observed in the negative group with no mixed biofilm structure. HE staining of surrounding tissue showed inflammatory cell infiltration in both groups, and neutrophils and lymphocytes were more in the positive group than in the negative group. There was no significant difference in the number of bacterial infections in heart and liver between the two groups ( >0.05). The number of bacterial infections in lung and kidney in the positive group was higher than that in negative group ( <0.05).

CONCLUSION

In the mixed infection of and , the ica operon may strengthen the structure of the biofilm and the spread of the biofilm , leading to increased inflammatory factors, and the bacteria are difficult to remove and persist.