Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Department of Materials Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
J Am Chem Soc. 2021 Oct 27;143(42):17441-17451. doi: 10.1021/jacs.1c06356. Epub 2021 Oct 15.
Surface layer proteins perform multiple functions in prokaryotic cells, including cellular defense, cell-shape maintenance, and regulation of import and export of materials. However, mimicking the complex and dynamic behavior of such two-dimensional biochemical systems is challenging, and hence research has so far focused mainly on the design and manipulation of the structure and functionality of protein assemblies in solution. Motivated by the new opportunities that dynamic surface layer proteins may offer for modern technology, we herein demonstrate that immobilization of coiled coil proteins onto an inorganic surface facilitates complex behavior, manifested by reversible chemical reactions that can be rapidly monitored as digital surface readouts. Using multiple chemical triggers as inputs and several surface characteristics as outputs, we can realize reversible switching and logic gate operations that are read in parallel. Moreover, using the same coiled coil protein monolayers for derivatization of nanopores drilled into silicon nitride membranes facilitates control over ion and mass transport through the pores, thereby expanding the applicability of the dynamic coiled coil system for contemporary stochastic biosensing applications.
表面层蛋白在原核细胞中执行多种功能，包括细胞防御、维持细胞形状以及调节物质的输入和输出。然而，模拟这种二维生化系统的复杂和动态行为具有挑战性，因此迄今为止的研究主要集中在设计和操纵溶液中蛋白质组装体的结构和功能上。受动态表面层蛋白可能为现代技术带来的新机遇的启发，我们在此证明，将螺旋蛋白固定在无机表面上有助于实现复杂的行为，表现为可作为数字表面读数快速监测的可逆化学反应。使用多种化学触发作为输入，并将几个表面特性作为输出，我们可以实现可并行读取的可逆开关和逻辑门操作。此外，使用相同的螺旋蛋白单层对氮化硅膜上钻取的纳米孔进行衍生化，有助于控制离子和质量通过孔的传输，从而扩展动态螺旋蛋白系统在当代随机生物传感应用中的适用性。
