Zhang Dan, Wang Chunfei, Wu Changfeng, Zhang Xuanjun
MOE Frontiers Science Center for Precision Oncology, Faculty of Health Sciences, University of Macau, Macau 999078, China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.
Nanomaterials (Basel). 2022 Apr 26;12(9):1468. doi: 10.3390/nano12091468.
Here, we confined fluorescent probes to solid nanochannels to construct nanosensors, which not only significantly improved the reusability of the molecular probes, but also achieved ion current and fluorescence dual gating for more reliable detection. The combination of optical and electrical modalities can provide comprehensive spatiotemporal information that can be used to elucidate the sensing mechanism within the nanochannel. As a proof-of-concept experiment, fluorescein isothiocyanate (FITC)−hydrazine (N2H4) was selected to modify nanochannels for the effective detection of Hg2+. Based on spirolactam opening tactics, the system synergistically alters the surface charge and fluorescence intensity in response to Hg2+, establishing a dual open state of current and fluorescence. The newly prepared nanosensor exhibited a fast response (<1 min), high sensitivity, and selectivity towards Hg2+. Importantly, the nanodevice could be recovered by simple N2H4 treatment. Such sensing behavior could be used to implement optoelectronic dual-output XOR logical gates under the management of Hg2+ and N2H4. This strategy is anticipated to find broad applications in other nanochannel-based systems for various sensing applications used for monitoring of pollutants, food additives, and biomolecules.
在这里，我们将荧光探针限制在固体纳米通道中以构建纳米传感器，这不仅显著提高了分子探针的可重复使用性，还实现了离子电流和荧光双门控，以实现更可靠的检测。光学和电学模式的结合可以提供全面的时空信息，可用于阐明纳米通道内的传感机制。作为概念验证实验，选择异硫氰酸荧光素（FITC）-肼（N2H4）修饰纳米通道以有效检测Hg2+。基于螺内酰胺开环策略，该系统协同响应Hg2+改变表面电荷和荧光强度，建立电流和荧光的双开放状态。新制备的纳米传感器对Hg2+表现出快速响应（<1分钟）、高灵敏度和选择性。重要的是，该纳米器件可以通过简单的N2H4处理回收。这种传感行为可用于在Hg2+和N2H4的管理下实现光电双输出异或逻辑门。预计该策略将在其他基于纳米通道的系统中广泛应用于各种传感应用，用于监测污染物、食品添加剂和生物分子。
