Kivistö A K, Vasenius H, Lindberg L A, Sandholm M
Invest Urol. 1978 Mar;15(5):412-5.
A functional test was developed to measure different adhesive properties of various Escherichia coli strains on the urinary tract epithelium. The test is based on initial incubation of everted canine ureteral segments in 2-hr cultures of E. coli (adsorption period), followed by sequential elutions in phosphate buffered saline. The release of bacteria (desorption rate) was measured from each elution by quantitative bacteriology. The function of the desorption assay was tested by showing different adhesive properties of various strains of E. coli. The strains isolated from infected canine urinary tracts showed high adhesion capacities superior to other strains of E. coli from different animal and/or tissue origin. This indicates a selective tissue and animal trophism determined by the strength of mucosal attachment. The test is also a valuable tool for measuring adhesion inhibiting substances in urine. The bulk of the adhesion inhibiting effect was localized in the urine ultrafiltrate.
开发了一种功能测试，以测量各种大肠杆菌菌株在尿路上皮上的不同粘附特性。该测试基于将外翻的犬输尿管段在大肠杆菌的2小时培养物中进行初始孵育（吸附期），然后在磷酸盐缓冲盐水中进行连续洗脱。通过定量细菌学测量每次洗脱中细菌的释放（解吸率）。通过显示各种大肠杆菌菌株的不同粘附特性来测试解吸测定的功能。从受感染的犬尿路中分离出的菌株显示出高于来自不同动物和/或组织来源的其他大肠杆菌菌株的高粘附能力。这表明由粘膜附着强度决定的选择性组织和动物嗜性。该测试也是测量尿液中粘附抑制物质的有价值工具。大部分粘附抑制作用定位于尿液超滤物中。
