College of Engineering, Northeast Agricultural University, Harbin 150030, PR China; Laboratory of Biomass Bio-chemical Conversion, GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
College of Engineering, Northeast Agricultural University, Harbin 150030, PR China.
Bioresour Technol. 2022 Jan;343:126118. doi: 10.1016/j.biortech.2021.126118. Epub 2021 Oct 12.
Low temperatures result in poor anaerobic digestion (AD). To investigate whether bioaugmentation can improve anaerobic co-digestion of cattle manure and corn straw at 20 °C, five different doses of methanogenic propionate-degrading culture (4%, 8%, 12%, 14%, and 16%) were added to batch AD systems to compare bioaugmentation performance. The results showed that the methane production of all the bioaugmented digesters was enhanced compared to the control, increasing 2.80-4.20-fold with digestion times (T) shorter by 11-22 d. The recommended dose for biogas production was 14%, and the recommended dose for the highest bioaugmentation efficiency of microbes was 4%. These improvements were due to the addition of methanogenic propionate-degrading culture, which alleviated volatile fatty acids (VFA) accumulation, especially that of acetate and propionate. Metagenomic sequencing analysis indicated that the increased proportion of propionate-oxidizing bacteria, syntrophic butyrate-oxidizing bacteria, and acetoclastic methanogens in bioaugmentation reactors may be responsible for better AD performance.
低温会导致厌氧消化（AD）效果不佳。为了研究生物强化是否可以提高 20°C 下牛粪和玉米秸秆的厌氧共消化，向批量 AD 系统中添加了五种不同剂量的产甲烷丙酸降解培养物（4%、8%、12%、14%和 16%），以比较生物强化性能。结果表明，与对照组相比，所有生物强化消化器的甲烷产量都得到了提高，消化时间（T）缩短了 11-22 天，产甲烷量增加了 2.80-4.20 倍。对于沼气生产，推荐剂量为 14%，对于微生物的最高生物强化效率，推荐剂量为 4%。这些改进是由于添加了产甲烷丙酸降解培养物，缓解了挥发性脂肪酸（VFA）的积累，特别是乙酸和丙酸的积累。宏基因组测序分析表明，生物强化反应器中丙酸氧化菌、丁酸共氧化菌和乙酸嗜甲烷菌的比例增加，可能是 AD 性能更好的原因。
