Southern Marine Science and Engineering Guangdong Larboratory, Zhanjiang, PR China; Institute of Modern Aquaculture Science and Engineering (IMASE) College of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Institute of Modern Aquaculture Science and Engineering (IMASE) College of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Fish Shellfish Immunol. 2021 Dec;119:339-346. doi: 10.1016/j.fsi.2021.10.013. Epub 2021 Oct 12.
Seeking out fish meal (FM) alternatives is an important requirement for aquaculture all over the world. And most practitioners believe that the plant protein is most potential for FM surrenal. While high plant protein feed caused some common problems in aquatic livestock: the absorption rate and growth rate are decreased, and even caused digestive tract inflammation. In present study, the inflence of high plant protien feed in Trachinotus ovatus was investigated using illumina HiSeqTM2000 based RNA-Seq. By comparing the two groups of cDNA libraries developed from high plant protien based diet or FM based diet fed T. ovatus livers, 836 unigenes were significantly upregulated, and 345 were significantly down regulated. KEGG analysis indicated that the differentially expressed genes (DEGs) are mainly metabolic-related genes. It was found that more than 28 DGEs beloned to the protein metabolism and absorption, lipid biosynthesis or other metabolic pathways. It indicated that high plant protein based diet had broad effects on metabolism on T. ovatus. There were also more DEGs belong to immune-related signaling pathways, include genes were involved in pathpathogen resistance and genes related to immunity system. These DEGs provided useful clues to explore the mechanisms that high plant protein based diet caused side effects on T. ovatus. These results improved our current understanding of the response of high plant protein based diet in T. ovatus, and outstanding the reasons of the side effect caused by high protein based diet.
寻找鱼粉（FM）替代品是全世界水产养殖业的重要要求。大多数从业者认为植物蛋白是替代 FM 的最有潜力的选择。然而，高蛋白饲料在水产养殖动物中会引起一些常见问题：吸收率和生长速度下降，甚至导致消化道炎症。在本研究中，使用基于 illumina HiSeqTM2000 的 RNA-Seq 技术研究了高蛋白饲料对卵形鲳鲹（Trachinotus ovatus）的影响。通过比较基于高蛋白饲料或 FM 喂养的卵形鲳鲹肝脏的两组 cDNA 文库，发现了 836 个显著上调的基因和 345 个显著下调的基因。KEGG 分析表明，差异表达基因（DEGs）主要与代谢相关。研究发现，超过 28 个 DGEs 属于蛋白质代谢和吸收、脂质生物合成或其他代谢途径。这表明高蛋白饲料对卵形鲳鲹的代谢有广泛的影响。还有更多的 DEGs 属于免疫相关信号通路，包括参与病原体抗性和免疫系统相关的基因。这些 DEGs 为探索高蛋白饲料对卵形鲳鲹产生副作用的机制提供了有用的线索。这些结果提高了我们对高蛋白饲料在卵形鲳鲹中的反应的理解，并突出了高蛋白饲料产生副作用的原因。
