Shanghai Laboratory Animal Research Center, Shanghai, China.
Shanghai Model Organisms Center, Inc., Shanghai, China.
Biochem Biophys Res Commun. 2021 Dec 3;581:12-19. doi: 10.1016/j.bbrc.2021.10.022. Epub 2021 Oct 10.
CD8 T cells play a critical role during adaptive immune response, which often change locations and expand or contract in numbers under different states. In the past, many attempts to develop CD8T cells that express luciferase in vivo have involved the use of viral transduction, which has drawbacks of hardly tracked via detection of luciferase signal in untouched natural states. Here, we generate a transgenic mouse model via CRISPR-mediated genome editing, C57BL/6-CD8a knock-in mice(CD8a-Aka mice), as a novel tool for non-invasive imaging of CD8 T cells, which expressed a highly sensitive luciferase-Akaluciferase. Our study offers a convenient and robust tool for understanding fundamental CD8 T cell biology in experimental applications and preclinical translational studies.
CD8 T 细胞在适应性免疫反应中发挥着关键作用，在不同状态下，它们经常改变位置并在数量上扩张或收缩。过去，许多试图在体内表达荧光素酶的 CD8T 细胞的尝试都涉及使用病毒转导，而这种方法很难在未受干扰的自然状态下通过检测荧光素酶信号来跟踪。在这里，我们通过 CRISPR 介导的基因组编辑生成了一种转基因小鼠模型，即 C57BL/6-CD8a 敲入小鼠（CD8a-Aka 小鼠），作为一种新型工具，可用于非侵入性成像 CD8 T 细胞，该细胞表达了一种高灵敏度的荧光素酶-Akaluciferase。我们的研究为在实验应用和临床前转化研究中理解基本的 CD8 T 细胞生物学提供了一种方便、强大的工具。
