基于随机结构光照明的超高分辨率拉曼显微镜：原理与可行性。

Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility.

机构信息

School of Electrical and Electronic Engineering, Yonsei University, Seoul 03722, South Korea.

Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

出版信息

J Chem Phys. 2021 Oct 14;155(14):144202. doi: 10.1063/5.0064082.

DOI:10.1063/5.0064082

PMID:34654313

Abstract

In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.

摘要

本文报告了一种基于拉曼显微镜的随机结构光照明化学成像方法，用于改善分子分布的图像分辨率。通过对测量的拉曼特征的时间和光谱特性生成随机结构基础图像，然后使用盲 SIM 算法进行结构光照明显微镜（SIM）。为了进行实验验证，从波长为 730-760nm 的范围内提取并重建了与罗丹明 6G（R6G）相对应的拉曼特征以及拉曼位移范围在 1096-1634cm 的拉曼特征，以构建 R6G 的图像。结果证实了该方法在概念上提高了图像分辨率，并暗示了将近两倍于衍射极限的超分辨率。