I. Department of Medicine, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, 55131, Mainz, Germany.
Institute of Forensic Medicine, Forensic Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, 55131, Mainz, Germany.
Sci Rep. 2021 Oct 15;11(1):20506. doi: 10.1038/s41598-021-00119-7.
Methylglyoxal (MGO) is a highly reactive dicarbonyl species that forms advanced glycation end products (AGEs). The binding of these AGEs to their receptor (RAGE) causes and sustains severe inflammation. Systemic inflammation is postulated to be a major driver in the progression of liver cirrhosis. However, the role of circulating MGO levels in liver cirrhosis remains unknown. In this study, we investigated the serum levels of two dicarbonyl species, MGO and glyoxal (GO) using tandem mass spectrometry (HPLC-MS/MS) and evaluated their association with disease severity. A total of 51 inpatients and outpatients with liver cirrhosis of mixed etiology and different disease stages were included. Elevated MGO levels were seen in an advanced stage of liver cirrhosis (p < 0.001). High MGO levels remained independently associated with impaired liver function, as assessed by the model for end-stage liver disease (MELD) (β = 0.448, p = 0.002) and acute decompensation (AD) (β = 0.345, p = 0.005) scores. Furthermore, MGO was positively correlated with markers of systemic inflammation (IL-6, p = 0.004) and the development of ascites (p = 0.013). In contrast, no changes were seen in GO serum levels. Circulating levels of MGO are elevated in advanced stages of liver cirrhosis and are associated with impaired liver function and liver-related parameters.
甲基乙二醛（MGO）是一种高度反应性的二羰基化合物，可形成晚期糖基化终产物（AGEs）。这些 AGE 与其受体（RAGE）结合会引起并持续严重的炎症。全身性炎症被认为是肝硬化进展的主要驱动因素。然而，循环 MGO 水平在肝硬化中的作用尚不清楚。在这项研究中，我们使用串联质谱法（HPLC-MS/MS）检测了两种二羰基化合物 MGO 和乙二醛（GO）的血清水平，并评估了它们与疾病严重程度的关系。共纳入 51 例混合病因和不同疾病阶段的肝硬化住院和门诊患者。晚期肝硬化患者的 MGO 水平升高（p<0.001）。高水平的 MGO 仍然与肝功能受损独立相关，这通过终末期肝病模型（MELD）评分（β=0.448，p=0.002）和急性失代偿（AD）评分（β=0.345，p=0.005）来评估。此外，MGO 与全身性炎症标志物（IL-6，p=0.004）和腹水形成（p=0.013）呈正相关。相比之下，GO 的血清水平没有变化。循环 MGO 水平在肝硬化晚期升高，并与肝功能受损和与肝脏相关的参数相关。
