Institute for Chronobiology, Foundation for Advancement of International Science (FAIS), 3-24-16 Kasuga, Tsukuba, Ibaraki, 305-0821, Japan.
Advanced Institute of Innovative Technology, Saitama University, Saitama, Japan.
Sci Rep. 2021 Oct 15;11(1):20543. doi: 10.1038/s41598-021-99753-4.
Although electric fields (EF) exert beneficial effects on animal wound healing, differentiation, cancers and rheumatoid arthritis, the molecular mechanisms of these effects have remained unclear about a half century. Therefore, we aimed to elucidate the molecular mechanisms underlying EF effects in Drosophila melanogaster as a genetic animal model. Here we show that the sleep quality of wild type (WT) flies was improved by exposure to a 50-Hz (35 kV/m) constant electric field during the day time, but not during the night time. The effect was undetectable in cryptochrome mutant (cry) flies. Exposure to a 50-Hz electric field under low nutrient conditions elongated the lifespan of male and female WT flies by ~ 18%, but not of several cry mutants and cry RNAi strains. Metabolome analysis indicated that the adenosine triphosphate (ATP) content was higher in intact WT than cry gene mutant strains exposed to an electric field. A putative magnetoreceptor protein and UV-A/blue light photoreceptor, CRYPTOCHROME (CRY) is involved in electric field (EF) receptors in animals. The present findings constitute hitherto unknown genetic evidence of a CRY-based system that is electric field sensitive in animals.
尽管电场 (EF) 对动物伤口愈合、分化、癌症和类风湿性关节炎有有益的影响，但这些影响的分子机制在半个世纪以来仍不清楚。因此，我们旨在阐明 EF 对黑腹果蝇的影响的分子机制，将其作为遗传动物模型。在这里，我们表明，在白天暴露于 50-Hz（35 kV/m）恒定电场中可以改善野生型（WT）果蝇的睡眠质量，但在夜间则不行。在隐色素突变体（cry）果蝇中，这种效应无法检测到。在低营养条件下暴露于 50-Hz 电场可使雄性和雌性 WT 果蝇的寿命延长约 18%，但对几种 cry 突变体和 cry RNAi 菌株则没有作用。代谢组学分析表明，与未暴露于电场的 cry 基因突变株相比，暴露于电场的完整 WT 菌株中的三磷酸腺苷（ATP）含量更高。一种假定的磁受体蛋白和 UV-A/蓝光光受体 CRYPTOCHROME (CRY) 参与动物的电场 (EF) 受体。本研究结果构成了迄今为止未知的基于 CRY 的系统在动物中对电场敏感的遗传证据。
