Ito Kumpei, Kawasaki Haruhisa, Suzuki Takahiro, Takahara Tsubasa, Ishida Norio
Institute of Chronobiology, Foundation for Advancement of International Science, Tsukuba, Japan.
Graduate School of Life and Environmental Sciences, Tsukuba University, Tsukuba, Japan.
Front Psychiatry. 2017 Jul 31;8:132. doi: 10.3389/fpsyt.2017.00132. eCollection 2017.
Parkinson's disease (PD) is the second most common neurodegenerative disease, and it is associated with sleep behavior disorders. In disease model, human α-synuclein A30P overexpressing flies (A30P PD model) have been shown for levy body aggregation and movement disorders. We measured sleep rhythms in the A30P PD model flies using the Activity Monitoring system and found that they develop sleep defects at 20 days after eclosion. Furthermore, the total amount of sleep is significantly reduced in middle-aged PD model flies and the reduction has been attributed to nighttime sleep. The number and length of sleep bouts also decreased in middle-aged A30P PD model flies. Feeding of the oriental traditional herbal medicines (Kampo), Kamikihito and Unkei-to significantly ameliorate the level of sleep defects in A30P PD model flies. The Kamikihito and Unkei-to recovered 60-min sleep bouts number in the A30P PD model flies to the level of young (5 days after eclosion) flies. Kamikihito recovered sleep both in wild-type and PD model flies. Unkei-to ameliorates not only sleep but also motor function in PD model flies. The data suggest that Kamikihito and Unkei-to might be useful for the sleep defects in human PD patients as well as healthy human.
帕金森病（PD）是第二常见的神经退行性疾病，且与睡眠行为障碍有关。在疾病模型中，过表达人α-突触核蛋白A30P的果蝇（A30P PD模型）已被证明存在路易小体聚集和运动障碍。我们使用活动监测系统测量了A30P PD模型果蝇的睡眠节律，发现它们在羽化后20天出现睡眠缺陷。此外，中年PD模型果蝇的总睡眠时间显著减少，且这种减少归因于夜间睡眠。中年A30P PD模型果蝇的睡眠周期数量和时长也有所减少。喂食东方传统草药（汉方）、加味逍遥散和温经汤可显著改善A30P PD模型果蝇的睡眠缺陷水平。加味逍遥散和温经汤使A30P PD模型果蝇60分钟的睡眠周期数量恢复到年轻果蝇（羽化后5天）的水平。加味逍遥散在野生型果蝇和PD模型果蝇中均能恢复睡眠。温经汤不仅能改善PD模型果蝇的睡眠，还能改善其运动功能。这些数据表明，加味逍遥散和温经汤可能对人类PD患者以及健康人的睡眠缺陷有用。
