Zurich University of Applied Sciences, Institute of Chemistry and Biotechnology, Natural Products and Phytopharmacy Research Group, Wädenswil, Switzerland.
Technical University Dresden, Faculty of Biology, Dresden, Germany.
Planta Med. 2021 Nov;87(14):1152-1166. doi: 10.1055/a-1647-2930. Epub 2021 Oct 15.
The genus (Lamiaceae) comprises around 150 species, of which many are popular herbal remedies in Mediterranean folk medicine. Already mentioned by Dioscorides and Theophrastus, the "ironwort" or "Greek mountain tea" has been receiving increased attention in recent years. A European Union herbal monograph and assessment report (HMPC) has been issued, covering the species and . This study presents results of a first pharmacognostic examination of the botanical and phytochemical differences among and between these emerging commercial species, and other, less studied species. An HPTLC method is proposed for normal phase separation of the species; this means applying two mobile phases on silica plates and subsequent derivatization with natural product reagent (NP/PEG) for visualization of phenolic compounds and anisaldehyde for a broader detection. With the help of selected reference compounds, a system suitability test was established for proper chromatographic separation. The method was applied to specimens from botanical gardens and commercial raw material in order to test its suitability for differentiation and authentication. The HPTLC analysis also includes, for the first time, and other less used species. The results might enable the development of a validated phytochemical fingerprint authentication procedure for quality assurance of Sideritis herba.
属（唇形科）包含约 150 个种，其中许多是地中海民间医学中流行的草药疗法。“铁线莲”或“希腊高山茶”已经被 Dioscorides 和 Theophrastus 提及，近年来受到越来越多的关注。已经发布了一份关于该属的欧盟草药专论和评估报告（HMPC），涵盖了 和 两个物种。本研究首次对这些新兴商业物种以及其他研究较少的物种之间和之间的植物学和植物化学差异进行了药代动力学检查。提出了一种用于正相分离的 HPTLC 方法；这意味着在硅胶板上应用两种流动相，然后用天然产物试剂（NP/PEG）进行衍生化以可视化酚类化合物，并用茴香醛进行更广泛的检测。通过使用选定的参考化合物，建立了系统适用性测试以实现适当的色谱分离。该方法适用于植物园和商业原料中的标本，以测试其用于区分和鉴定的适用性。HPTLC 分析还首次包括 和其他使用较少的 物种。结果可能有助于为 sideritis herba 的质量保证开发经过验证的植物化学指纹鉴定程序。
