不同 PVP 液孵育时间对精子参数和 DNA 完整性的评估。
Evaluation of Sperm Parameters and DNA Integrity Following Different Incubation Times in PVP Medium.
机构信息
1- Abortion research center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Urol J. 2021 Oct 13;19(3):232-237. doi: 10.22037/uj.v18i.6936.
PURPOSE
Polyvinylpyrrolidone (PVP) is a chemical material used in intracytoplasmic sperm injection (ICSI) program. The aim of this study was to investigate the ideal time that sperm can be safely incubated in PVP with less structure and DNA damage.
METHOD
Thirty-one Oligoasthenoteratospermia (OAT) samples were used. Sperm samples were prepared by discontinuous density-gradients method and incubated in 10% PVP at different time intervals (0, 5, 10, 15, 20, and 30 min). The effect of PVP was assessed on sperm DNA fragmentation and viability via SCD assay and Eosin-nigrosin staining respectively.
RESULTS
Data showed there was a significant increase in sperm DNA fragmentation at 10 min compared to 0 min. The viability rate also significantly reduced at 10 min compared to 0 min.
CONCLUSION
As a result, sperm samples could be incubated with PVP for less than 10 min. While prolonged incubation may significantly damage the sperm DNA integrity and viability.
目的
聚乙烯吡咯烷酮（PVP）是一种用于胞浆内单精子注射（ICSI）程序的化学物质。本研究旨在探讨精子在 PVP 中安全孵育的理想时间，以减少结构和 DNA 损伤。
方法
使用 31 份少精弱精症（OAT）样本。通过不连续密度梯度法制备精子样本，并在 10%PVP 中孵育不同时间间隔（0、5、10、15、20 和 30 分钟）。通过 SCD 分析和伊红-苯胺黑染色分别评估 PVP 对精子 DNA 碎片化和活力的影响。
结果
数据显示，与 0 分钟相比，10 分钟时精子 DNA 碎片化显著增加。与 0 分钟相比，10 分钟时精子活力也显著降低。
结论
因此，精子样本可以在 PVP 中孵育不到 10 分钟。而长时间孵育可能会显著损害精子 DNA 的完整性和活力。
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