Mohammadi Forogh, Karimi-Dehkordi Maryam, Pouriayevali Farnaz
Department of Veterinary, Agriculture Faculty, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Int J Reprod Biomed. 2024 Jul 8;22(5):383-394. doi: 10.18502/ijrm.v22i5.16439. eCollection 2024 May.
() causes sterility by affecting the reproductive system. Vitamin B improves sperm quantity and function.
Vitamin B protection against adverse effects was investigated.
40 C57 male mice (6 wk) were randomly assigned to 4 equal groups (n = 10) including, group 1 (control without any intervention), group 2 (H), 3 (HP), and 4 (HB) received 1 10 colony forming unit (CFU) of , 1 10 CFU of +phosphate buffered saline, 1 10 CFU of 50 g/kg vitamin B intraperitoneally, respectively. In the induction groups, the was orogasterically injected 3 times with 1 cc phosphate buffered saline throughout the day. Then testicular metrics, sperm motility, viability, quantity, and shape, plasma levels of malondialdehyde (MDA), superoxide dismutase, glutathione peroxidase, and total antioxidant capacity were measured. Also, testicular-tissue changes were examined using Johnson scores, tubular differentiation index, and spermatogenesis index. Vitamin B, homocysteine, and testosterone serum levels were examined.
The results showed a significantly lower Johnson score, tubular differentiation index, and spermatogenesis index, and serum level of testosterone and homocysteine as well as a higher MDA level in the H and HP groups than the HB group (p 0.05). In contrast, the highest superoxide dismutase and glutathione peroxidase enzymes activity and total antioxidant capacity as well as the lowest serum level of MDA were found in the HB group compared to other groups (p 0.05).
Vitamin B increased antioxidant enzyme activity, enhanced sperm parameters, and decreased injury to testicular tissue. It can be used as a potent antioxidant in reducing testicular damage induced by .
（）通过影响生殖系统导致不育。维生素B可改善精子数量和功能。
研究维生素B对不良影响的保护作用。
将40只6周龄的C57雄性小鼠随机分为4组，每组10只，分别为：第1组（未干预对照组）、第2组（H组）、第3组（HP组）和第4组（HB组），分别腹腔注射1×10菌落形成单位（CFU）的（）、1×10 CFU的（）+磷酸盐缓冲盐水、1×10 CFU的（）+50μg/kg维生素B。在诱导组中，全天经口胃内注射3次1 cc磷酸盐缓冲盐水的（）。然后测量睾丸指标、精子活力、存活率、数量和形态、血浆丙二醛（MDA）水平、超氧化物歧化酶、谷胱甘肽过氧化物酶和总抗氧化能力。此外，使用约翰逊评分、小管分化指数和生精指数检查睾丸组织变化。检测维生素B、同型半胱氨酸和睾酮血清水平。
结果显示，与HB组相比，H组和HP组的约翰逊评分、小管分化指数和生精指数以及睾酮和同型半胱氨酸血清水平显著降低，MDA水平更高（p<0.05）。相比之下，与其他组相比，HB组的超氧化物歧化酶和谷胱甘肽过氧化物酶活性以及总抗氧化能力最高，MDA血清水平最低（p<0.05）。
维生素B可提高抗氧化酶活性，增强精子参数，减少睾丸组织损伤。它可作为一种有效的抗氧化剂用于减少（）诱导的睾丸损伤。
