Aukhil I, Simpson D M, Suggs C, Pettersson E
J Clin Periodontol. 1986 Oct;13(9):862-8. doi: 10.1111/j.1600-051x.1986.tb02244.x.
The present study was designed to test the hypothesis that during regeneration of cementum, the progenitor cells from periodontal ligament must come in contact with root dentin in order to differentiate into cementoblasts. After reflecting mucoperiosteal flaps, fenestration wounds were made in the buccal cortical plates of mandibular canines in 6 beagle dogs. The exposed root surfaces were curretted to remove all cementum. The exposed root surface on one side was demineralized with citric acid while the contralateral wound had saline treatment. The exposed root surfaces were then dried and pieces of Nuclepore membrane (pore size 0.1 mu) were attached to part of the exposed root surface to prevent contact of progenitor cells with root dentin. The fenestration wounds were then covered with Millipore filter to facilitate the population of wounds by progenitor cells from the periodontal ligament. Histologic analysis was performed after 3 months of healing. In specimens where the Nuclepore membrane had remained attached to root dentin, no new cementum was seen over the membrane. At the borders of the wounds and in specimens where the Nuclepore membrane had detached from root dentin, new connective tissue attachment was consistently seen. Also, root resorption was very rarely observed in both the acid-treated and control specimens. The present findings suggest that contact with root dentin may be necessary for progenitor cell differentiation into formative cells like cementoblasts.
在牙骨质再生过程中，牙周膜祖细胞必须与牙根牙本质接触才能分化为成牙骨质细胞。在6只比格犬的下颌尖牙颊侧皮质板上翻起黏骨膜瓣后，制作开窗创口。将暴露的牙根表面刮治以去除所有牙骨质。一侧暴露的牙根表面用柠檬酸脱矿，而对侧创口用生理盐水处理。然后将暴露的牙根表面干燥，并将微孔滤膜（孔径0.1微米）片附着于部分暴露的牙根表面，以防止祖细胞与牙根牙本质接触。然后用微孔滤膜覆盖开窗创口，以利于牙周膜祖细胞聚集于创口。愈合3个月后进行组织学分析。在微孔滤膜仍附着于牙根牙本质的标本中，膜上未见新的牙骨质形成。在创口边缘以及微孔滤膜已从牙根牙本质脱离的标本中，始终可见新的结缔组织附着。此外，在酸处理组和对照组标本中均很少观察到牙根吸收。本研究结果提示，祖细胞分化为成牙骨质细胞等形成性细胞可能需要与牙根牙本质接触。
