Harshan Sruthy, Lobo Lancelot, Kumari Suchetha, Shetty Shilpa
Department of General Surgery, K S Hegde Medical Academy of Nitte University, Mangalore, Karnataka India.
Department of Biochemistry, K S Hegde Medical Academy of Nitte University, Mangalore, Karnataka India.
Indian J Surg Oncol. 2021 Sep;12(3):460-464. doi: 10.1007/s13193-021-01373-x. Epub 2021 Jul 8.
Worldwide, carcinoma of the oesophagus is the sixth leading reason for deaths due to malignancy. MicroRNAs (MiRs) are a cluster of small non-coding RNAs that have potent stability in stored RNA samples. This strengthens their potential as biomarkers. This study aimed to isolate MiR 21 from serum samples of carcinoma oesophagus patients and healthy subjects for determining its strength of association in various stages of the carcinoma oesophagus. This is a case-control study with 1:1 matched cases and controls. We included 40 patients of carcinoma oesophagus and 40 healthy individuals. Serum separated from venous blood samples of cases and controls was stored at - 80 °C. Total RNA was extracted, and the real-time polymerase chain reaction was used to measure the serum MiR 21. On statistical comparison, we found a marked increase in MiR 21 in cases of carcinoma oesophagus when compared with the controls (p < 0.0001). This study also showed a steady rise in the MiR 21 levels with the disease severity (p < 0.0001). Based on the result of this study, we conclude that MiR 21 levels can be used as a novel marker for assessing disease severity in carcinoma oesophagus.
在全球范围内，食管癌是因恶性肿瘤导致死亡的第六大主要原因。微小RNA（miRs）是一类小型非编码RNA，在储存的RNA样本中具有很强的稳定性。这增强了它们作为生物标志物的潜力。本研究旨在从食管癌患者和健康受试者的血清样本中分离出miR 21，以确定其在食管癌各个阶段的关联强度。这是一项病例对照研究，病例与对照按1:1匹配。我们纳入了40例食管癌患者和40名健康个体。将病例组和对照组静脉血样本分离出的血清储存在-80°C。提取总RNA，并使用实时聚合酶链反应测量血清miR 21。经统计学比较，我们发现与对照组相比，食管癌病例中的miR 21显著增加（p < 0.0001）。本研究还表明，miR 21水平随疾病严重程度稳步上升（p < 0.0001）。基于本研究结果，我们得出结论，miR 21水平可作为评估食管癌疾病严重程度的新型标志物。
