Hezova Renata, Kovarikova Alena, Srovnal Josef, Zemanova Milada, Harustiak Tomas, Ehrmann Jiri, Hajduch Marian, Svoboda Marek, Sachlova Milana, Slaby Ondrej
Molecular Oncology II - Solid Cancers, Molecular Medicine Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, 656 53, Czech Republic.
Diagn Pathol. 2015 Apr 28;10:42. doi: 10.1186/s13000-015-0280-6.
Esophageal cancer is the malignant tumor with very poor prognosis and increasing incidence often diagnosed at very late stage, so the prognosis of affected patients is unsatisfactory, despite the development of therapeutic option such as surgery, chemotherapy and radiotherapy. Consequently, there is a great need for biomarkers to allow a tailored multimodality approach with increased efficiency. Altered expression of microRNAs has been reported in wide range of malignancies, including esophageal cancer. The aim of this study was to examine the expression levels of candidate microRNAs in esophageal cancer and evaluate their diagnostic and prognostic potential.
Using quantitative real-time PCR, expression levels of 9 candidate microRNAs were examined in 62 tissue samples, 23 esophageal adenocarcinomas, 22 esophageal squamous cell carcinomas and 17 adjacent esophageal mucosa samples. MicroRNA expression levels were further analyzed in regards to clinico-pathological features of esophageal cancer patients. We observed significantly decreased levels of miR-203 and increased levels of miR-21 in adenocarcinoma tissues when compared to normal mucosa. MiR-29c and miR-148 indicated good ability to distinguish between histological subtypes of esophageal cancer. MiR-203 and miR-148 were linked to disease-free survival and overall survival in esophageal adenocarcinoma patients, and miR-148 also in esophageal squamous cell carcinoma patients.
Our data suggest that altered expression of miR-21, miR-29c, miR-148 and miR-203 are related to neoplastic transformation and progression of the disease and these microRNAs could serve as a potential diagnostic and prognostic biomarkers in esophageal cancer.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4646922201567057.
食管癌是一种预后很差且发病率不断上升的恶性肿瘤，通常在很晚的阶段才被诊断出来，因此，尽管有手术、化疗和放疗等治疗手段，但受影响患者的预后仍不令人满意。因此，迫切需要生物标志物，以便采用更高效的个性化多模式治疗方法。据报道，包括食管癌在内的多种恶性肿瘤中都存在微小RNA表达的改变。本研究的目的是检测食管癌中候选微小RNA的表达水平，并评估其诊断和预后潜力。
使用定量实时聚合酶链反应，在62个组织样本（23个食管腺癌、22个食管鳞状细胞癌和17个相邻食管黏膜样本）中检测了9种候选微小RNA的表达水平。还根据食管癌患者的临床病理特征对微小RNA表达水平进行了进一步分析。与正常黏膜相比，我们观察到腺癌组织中miR-203水平显著降低，miR-21水平升高。miR-29c和miR-148显示出良好的区分食管癌组织学亚型的能力。miR-203和miR-148与食管腺癌患者的无病生存期和总生存期相关，miR-148也与食管鳞状细胞癌患者的生存期相关。
我们的数据表明，miR-21、miR-29c、miR-148和miR-203表达的改变与肿瘤的发生和疾病进展有关，这些微小RNA可作为食管癌潜在的诊断和预后生物标志物。
本文的虚拟切片可在此处找到：http://www.diagnosticpathology.diagnomx.eu/vs/4646922201567057。
