Gomułka Krzysztof, Wrześniak Marta, Mędrala Wojciech, Panaszek Bernard
Department of Internal Medicine, Pneumology and Allergology, Medical University of Wroclaw, Wroclaw, Poland.
Postepy Dermatol Alergol. 2021 Aug;38(4):608-610. doi: 10.5114/ada.2021.108910. Epub 2021 Sep 17.
Basophils are one of the main target cells in chronic spontaneous urticaria (CSU). If cells present higher susceptibility to production and degranulation of pro-inflammatory factors, priming may be associated with severity of symptoms and therapy ineffectiveness.
To evaluate the spontaneous state of increase in basophil activity and their priming profile in patients with CSU.
The study sample included 22 patients diagnosed with CSU and 20 healthy volunteers without either allergy symptoms or CSU. In this study, we evaluate the presence of CD63 and CD63+CD203c at basophils surface by flow cytometry test (basophil activation test - BAT).
We found that the percentage of activated basophils was higher in patients with CSU than in the control group and this difference was statistically significant ( < 0.05).
Our results indicate a greater degree of basophils activation in patients with CSU in remission than in the control group; it might be useful for identification of patients with predominance of the autoimmune variant of CSU and typing patients responding (responders) and refractory (non-responders) to treatment with antihistamines.
嗜碱性粒细胞是慢性自发性荨麻疹（CSU）的主要靶细胞之一。如果细胞对促炎因子的产生和脱颗粒表现出更高的易感性，致敏可能与症状严重程度及治疗无效有关。
评估CSU患者嗜碱性粒细胞活性增加的自发状态及其致敏情况。
研究样本包括22例诊断为CSU的患者和20名无过敏症状或CSU的健康志愿者。在本研究中，我们通过流式细胞术检测（嗜碱性粒细胞活化试验 - BAT）评估嗜碱性粒细胞表面CD63和CD63 + CD203c的存在情况。
我们发现CSU患者中活化嗜碱性粒细胞的百分比高于对照组，且这种差异具有统计学意义（<0.05）。
我们的结果表明，缓解期CSU患者的嗜碱性粒细胞活化程度高于对照组；这可能有助于识别以CSU自身免疫变体为主的患者，以及区分对抗组胺药治疗有反应（反应者）和难治（无反应者）的患者类型。
