Department of Dermatology, Southwest Hospital, Army Medical University, Chongqing, China,
Department of Dermatology, Southwest Hospital, Army Medical University, Chongqing, China.
Int Arch Allergy Immunol. 2023;184(11):1126-1134. doi: 10.1159/000531966. Epub 2023 Aug 21.
Allergen-specific IgE (sIgE) sensitization exists in a considerable fraction of chronic spontaneous urticaria (CSU) patients. Basophils have been implicated in the pathogenesis of CSU. This paper aimed to explore the relationship between allergic sensitization and basophil reactivity in CSU and the possible underlying mechanism.
Basophil-enriched leukocytes were isolated from the peripheral blood of 76 CSU patients and 9 healthy controls. Basophil CD63 and FcεRIα (the alpha subunit of the high-affinity IgE receptor) expression in the blood samples with various house dust mite (HDM)-sIgE levels were determined by flow cytometry. Basophil reactivity and SHIP-1 (a molecule related to the IgE/FcεRI signaling pathway) expression were analyzed after stimulation with an HDM allergen or other stimuli.
HDM-sIgEstrong positive (≥3.5 kU/L) CSU patients had a significantly higher mean percentage of basophil CD63 and higher baseline levels of FcεRIα expressed by basophils than HDM-sIgEnormal (<0.35 kU/L) CSU patients and healthy controls; the same went for total serum IgE. After stimulation with Dermatophagoides pteronyssinus peptidase 1 (Derp1) alone or together with Derp1-sIgE, the stimulation index of CD63 and levels of FcεRIα expressed by basophils in HDM-sIgEstrong positive CSU patients were significantly higher than those in HDM-sIgEnormal CSU patients and healthy controls. Significantly more SHIP-1 mRNA expression in HDM-sIgEstrong positive CSU patients was induced after the combined stimulation in comparison to other subjects.
CSU patients with higher HDM-sIgE levels (≥3.5 kU/L) may have higher CD63 and FcεRIα expression on peripheral blood basophils. Peripheral blood basophils in these CSU patients are more responsive to HDM allergen stimulation. Higher HDM-sIgE levels among CSU patients may implicate higher basophil reactivity.
变应原特异性 IgE（sIgE）致敏存在于相当一部分慢性自发性荨麻疹（CSU）患者中。嗜碱性粒细胞被认为与 CSU 的发病机制有关。本文旨在探讨 CSU 中过敏致敏与嗜碱性粒细胞反应性之间的关系及可能的潜在机制。
从 76 例 CSU 患者和 9 例健康对照者的外周血中分离出嗜碱性粒细胞富集白细胞。通过流式细胞术检测不同屋尘螨（HDM）-sIgE 水平血液样本中嗜碱性粒细胞 CD63 和 FcεRIα（高亲和力 IgE 受体的α亚基）的表达。分析 HDM 过敏原或其他刺激物刺激后嗜碱性粒细胞反应性和 SHIP-1（与 IgE/FcεRI 信号通路相关的分子）的表达。
HDM-sIgE 强阳性（≥3.5 kU/L）的 CSU 患者的嗜碱性粒细胞 CD63 的平均百分比明显更高，嗜碱性粒细胞表达的 FcεRIα基础水平也高于 HDM-sIgE 正常（<0.35 kU/L）的 CSU 患者和健康对照者；总血清 IgE 也是如此。单独或与 Derp1-sIgE 一起刺激 Derp1 后，HDM-sIgE 强阳性 CSU 患者的嗜碱性粒细胞 CD63 刺激指数和 FcεRIα表达水平明显高于 HDM-sIgE 正常的 CSU 患者和健康对照者。与其他组相比，HDM-sIgE 强阳性 CSU 患者在联合刺激后诱导的 SHIP-1 mRNA 表达明显更多。
HDM-sIgE 水平较高（≥3.5 kU/L）的 CSU 患者外周血嗜碱性粒细胞的 CD63 和 FcεRIα表达可能更高。这些 CSU 患者的外周血嗜碱性粒细胞对 HDM 过敏原刺激的反应性更高。CSU 患者的 HDM-sIgE 水平较高可能意味着嗜碱性粒细胞反应性较高。
