Honarmand Tamizkar Kasra, Badrlou Elham, Aslani Termeh, Brand Serge, Arsang-Jang Shahram, Ghafouri-Fard Soudeh, Taheri Mohammad
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Front Mol Neurosci. 2021 Sep 28;14:747785. doi: 10.3389/fnmol.2021.747785. eCollection 2021.
Autism spectrum disorder (ASD) is a long-standing neurodevelopmental condition with prominent effects on social behavior of affected children. This disorder has been linked with neuroinflammatory responses. NF-κB has been shown to affect these responses in the orbitofrontal cortex of patients with ASD, thus being implicated in the pathogenesis of ASD. We measured expression of some NF-κB-associated lncRNAs and mRNAs ( and ) in the peripheral blood of ASD kids vs. healthy children. Expression quantities of and were meaningfully higher in ASD cases compared with healthy kids (Posterior Beta = 1.402, value < 0.0001; Posterior Beta = 2.959, value < 0.0001; Posterior Beta = 0.882, value = 0.012; Posterior Beta = 1.461, value < 0.0001; Posterior Beta = 0.541, value = 0.043, respectively). The Bonferroni corrected values for these lncRNAs remained significant except for and . Expression levels of other genes were not considerably different between cases and controls. Expressions of and were correlated with age of ASD patients ( < 0.0001). Among ASD cases, the most robust correlation has been detected between and ( = 0.87, < 0.0001). Expression of none of genes has been correlated with age of healthy children. Among this group of children, expression levels of and were robustly correlated ( = 0.83, < 0.0001). had the greatest AUC value (AUC = 0.857), thus the best diagnostic power among the assessed genes. ranked the second position in this regard (AUC = 0.757). Thus, NF-κB-associated lncRNAs might partake in the pathogenesis of ASD.
自闭症谱系障碍（ASD）是一种长期存在的神经发育疾病，对受影响儿童的社会行为有显著影响。这种疾病与神经炎症反应有关。研究表明，NF-κB会影响ASD患者眶额皮质中的这些反应，因此与ASD的发病机制有关。我们测量了ASD儿童与健康儿童外周血中一些与NF-κB相关的长链非编码RNA（lncRNA）和信使核糖核酸（mRNA）的表达。与健康儿童相比，ASD病例中[具体基因1]和[具体基因2]的表达量显著更高（后验β = 1.402，P值<0.0001；后验β = 2.959，P值<0.0001；后验β = 0.882，P值 = 0.012；后验β = 1.461，P值<0.0001；后验β = 0.541，P值 = 0.043）。除了[具体基因3]和[具体基因4]外，这些lncRNA经Bonferroni校正后的P值仍具有显著性。病例组和对照组之间其他基因的表达水平没有显著差异。[具体基因5]和[具体基因6]的表达与ASD患者的年龄相关（P<0.0001）。在ASD病例中，检测到[具体基因5]和[具体基因6]之间的相关性最强（r = 0.87，P<0.0001）。没有一个基因的表达与健康儿童的年龄相关。在这组儿童中，[具体基因7]和[具体基因8]的表达水平相关性很强（r = 0.83，P<0.0001）。[具体基因9]的曲线下面积（AUC）值最大（AUC = 0.857），因此在所评估的基因中诊断能力最佳。[具体基因10]在这方面排名第二（AUC = 0.757）。因此，与NF-κB相关的lncRNA可能参与了ASD的发病机制。
