Man Kee-Ming, Lee Yu-Chen, Chen Ying-I, Chen Yung Hsiang, Chang Shih Liang, Huang Chieh-Chen
Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da Yeh University, Changhua, Taiwan.
Evid Based Complement Alternat Med. 2021 Oct 6;2021:5852599. doi: 10.1155/2021/5852599. eCollection 2021.
In rats with 2-deoxy-2-(3-(methyl-3-nitrosoureido)-d-glucopyranose streptozotocin- (STZ-) induced insulin-dependent diabetes (IDDM), continuous 15 Hz electrical stimulation at bilateral ST36 acupoints for 30 and 60 minutes has been shown to prevent hyperglycemia. We hypothesized that the mechanism of action in STZ-induced IDDM rats is that electrical stimulation at bilateral ST36 acupoints is effective in improving insulin receptor substrate type 1 (IRS-1) and glucose transporter type 4 (GLUT4) protein expressions associated with counteracting both plasma glucose and free fatty acid (FFA) levels during isoflurane anesthesia. In this study, twenty-six healthy male Wistar rats, weighing 250-350 g and aged 8-10 weeks were tested. Rats in the experimental electroacupuncture (EA) group ( = 13) received 15 Hz electrical stimulation at bilateral ST 36 acupoints for 30 and 60 minutes. Rats in the control group ( = 13) were handled but not subjected to the stimulation treatment. In both IDDM and normal Wistar rats, we observed a negative change in plasma glucose levels when rats were given the EA treatment, but a positive change in plasma glucose without EA treatment relative to baseline. Within the IDDM group, a negative change in FFA levels was observed when rats were given the EA treatment, while a positive change in the FFA level was shown without the EA treatment. In the expressed protein signals, we found a significant elevation in both GLUT4 and IRS-1 proteins in the IDDM group treated by EA. Moreover, we found a significant mean difference between GLUT4 and IRS-1 protein expression levels relative to -actin. Our findings suggested that EA at bilateral ST36 acupoints could serve as an effective strategy for lowering plasma glucose by decreasing free fatty acid levels and improving the expression of IRS-1 and GLUT4 proteins in a STZ-IDDM rat model during isoflurane anesthesia.
在链脲佐菌素（STZ）诱导的胰岛素依赖型糖尿病（IDDM）大鼠中，对双侧足三里穴进行连续15Hz电刺激30分钟和60分钟已被证明可预防高血糖。我们推测，在STZ诱导的IDDM大鼠中，其作用机制是在异氟烷麻醉期间，双侧足三里穴的电刺激可有效改善胰岛素受体底物1型（IRS-1）和葡萄糖转运蛋白4型（GLUT4）的蛋白表达，从而抵消血糖和游离脂肪酸（FFA）水平。在本研究中，对26只体重250 - 350g、年龄8 - 10周的健康雄性Wistar大鼠进行了测试。实验性电针（EA）组（n = 13）的大鼠在双侧足三里穴接受15Hz电刺激30分钟和60分钟。对照组（n = 13）的大鼠接受了处理，但未进行刺激治疗。在IDDM大鼠和正常Wistar大鼠中，我们观察到，接受EA治疗的大鼠血糖水平呈负向变化，而未接受EA治疗的大鼠血糖水平相对于基线呈正向变化。在IDDM组中，接受EA治疗的大鼠FFA水平呈负向变化，而未接受EA治疗的大鼠FFA水平呈正向变化。在表达的蛋白信号中，我们发现EA治疗的IDDM组中GLUT4和IRS-1蛋白均显著升高。此外，相对于β-肌动蛋白，我们发现GLUT4和IRS-1蛋白表达水平存在显著的平均差异。我们的研究结果表明，在异氟烷麻醉期间，对双侧足三里穴进行电针治疗可作为一种有效的策略，通过降低游离脂肪酸水平和改善IRS-1和GLUT4蛋白的表达来降低STZ-IDDM大鼠模型的血糖水平。
