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监测仓鼠和小鼠的群体活动作为评估COVID-19进展、康复及rVSV-ΔG-Spike疫苗接种效果的新工具

Monitoring Group Activity of Hamsters and Mice as a Novel Tool to Evaluate COVID-19 Progression, Convalescence, and rVSV-ΔG-Spike Vaccination Efficacy.

作者信息

Melamed Sharon, Politi Boaz, Grauer Ettie, Achdout Hagit, Aftalion Moshe, Gur David, Tamir Hadas, Yahalom-Ronen Yfat, Maimon Shlomy, Yitzhak Efi, Weiss Shay, Rosner Amir, Erez Noam, Yitzhaki Shmuel, Shapira Shmuel C, Paran Nir, Mamroud Emanuelle, Vagima Yaron, Israely Tomer

机构信息

Israel Institute for Biological Research (IIBR), Ness-Ziona, Israel.

出版信息

Front Bioeng Biotechnol. 2021 Oct 1;9:737627. doi: 10.3389/fbioe.2021.737627. eCollection 2021.

DOI:10.3389/fbioe.2021.737627
PMID:34660558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8517107/
Abstract

The COVID-19 pandemic initiated a worldwide race toward the development of treatments and vaccines. Small animal models included the Syrian golden hamster and the K18-hACE2 mice infected with SARS-CoV-2 to display a disease state with some aspects of human COVID-19. A group activity of animals in their home cage continuously monitored by the HCMS100 (Home cage Monitoring System 100) was used as a sensitive marker of disease, successfully detecting morbidity symptoms of SARS-CoV-2 infection in hamsters and in K18-hACE2 mice. COVID-19 convalescent hamsters rechallenged with SARS-CoV-2 exhibited minor reduction in group activity compared to naive hamsters. To evaluate the rVSV-ΔG-spike vaccination efficacy against SARS-CoV-2, we used the HCMS100 to monitor the group activity of hamsters in their home cage. A single-dose rVSV-ΔG-spike vaccination of the immunized group showed a faster recovery than the nonimmunized infected hamsters, substantiating the efficacy of rVSV-ΔG-spike vaccine. HCMS100 offers nonintrusive, hands-free monitoring of a number of home cages of hamsters or mice modeling COVID-19.

摘要

新冠疫情引发了一场全球范围内研发治疗方法和疫苗的竞赛。小动物模型包括叙利亚金黄地鼠和感染了新冠病毒的K18-hACE2小鼠，以展现出具有人类新冠肺炎某些特征的疾病状态。通过HCMS100（笼内监测系统100）持续监测动物在其笼内的群体活动，将其用作疾病的敏感指标，成功检测到了地鼠和K18-hACE2小鼠感染新冠病毒的发病症状。再次感染新冠病毒的新冠康复地鼠与未感染过的地鼠相比，群体活动略有减少。为评估rVSV-ΔG-刺突疫苗对新冠病毒的接种效果，我们使用HCMS100监测地鼠在其笼内的群体活动。免疫组单剂量接种rVSV-ΔG-刺突疫苗后，恢复速度比未免疫的感染地鼠更快，证实了rVSV-ΔG-刺突疫苗的效果。HCMS100提供了对多个模拟新冠肺炎的地鼠或小鼠笼进行非侵入式、无需人工操作的监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/060af0355ced/fbioe-09-737627-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/91d1b0112279/fbioe-09-737627-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/987c86222715/fbioe-09-737627-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/060af0355ced/fbioe-09-737627-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/91d1b0112279/fbioe-09-737627-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/36f7ed601ff7/fbioe-09-737627-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/73e282a488da/fbioe-09-737627-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/987c86222715/fbioe-09-737627-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a5/8517107/060af0355ced/fbioe-09-737627-g005.jpg

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K18-hACE2 mice develop respiratory disease resembling severe COVID-19.
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