Kim Eun-Bi, Kim Myung Joon, Hwang InSeo, Park Hye-Min, Lee Sang Heon, Kim Hyun-Woo
Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, Republic of Korea.
Department of Oceanography, Pusan National University, Busan, Republic of Korea.
Mitochondrial DNA B Resour. 2021 Oct 14;6(11):3184-3185. doi: 10.1080/23802359.2021.1945503. eCollection 2021.
The Japanese sea lion, , is an extinct pinniped species, which had inhabited along the coast of the Japanese archipelago and Korean peninsula. Its mitochondrial genome was determined by the assembly of PCR amplicons from the skeletal remains excavated from the Ulleungdo, South Korea. The whole mitogenome was 16,698 bp in length, which encoded 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNA), 2 ribosomal RNAs (rRNA), an origin of light-strand replication (OL), and a control region (D-loop). Unusual start codons were identified in ND2 (ATA), ND3 (ATA), and ND5 (ATC), while COIII, ND3, and ND4 were terminated with an incomplete stop codon (T-/TA-). Phylogenetic analysis showed that . was a sister species to . with 98.61% nucleotide sequence identity among 11 pinniped species in the infraorder Pinnipedia, which supported the previous results. The complete mitochondrial genome sequence of . would be valuable information for its restoration and the evolutional understandings of pinniped species in the Pacific Ocean.
日本海狮是一种已灭绝的鳍足类物种，曾栖息在日本列岛和朝鲜半岛的海岸沿线。其线粒体基因组是通过对从韩国郁陵岛挖掘出的骨骼残骸的聚合酶链式反应扩增产物进行组装来确定的。整个线粒体基因组长度为16,698 bp，编码13个蛋白质编码基因（PCGs）、22个转运RNA（tRNA）、2个核糖体RNA（rRNA）、一个轻链复制起点（OL）和一个控制区（D环）。在ND2（ATA）、ND3（ATA）和ND5（ATC）中发现了不寻常的起始密码子，而COIII、ND3和ND4则以不完全终止密码子（T-/TA-）结束。系统发育分析表明，在鳍足亚目11种鳍足类物种中，日本海狮与北海狮是姐妹物种，核苷酸序列同一性为98.61%，这支持了先前的结果。日本海狮完整的线粒体基因组序列对于其恢复以及对太平洋鳍足类物种进化的理解将是有价值的信息。
