Fu'adil Amin Muhammad Hilman, Tabassum Nazia, Kim Ah-Ran, Lee Dae-Sung, Kim Hyun-Woo
Industrial Convergence Bionix Engineering, Pukyong National University, Busan, Republic of Korea.
Department of Biology, Faculty of Science and Technology, Advance Tropical Biodiversity, Genomics, and Conservation Research Group, Universitas Airlangga, Surabaya, Indonesia.
Mitochondrial DNA B Resour. 2020 Dec 24;5(4):3817-3818. doi: 10.1080/23802359.2020.1840943.
We applied next-generation sequencing (NGS) method to construct the complete mitochondrial genome of . The obtained mitogenome of (16,590 bp) exhibited a typical structure harboring 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one control regions (D-loop). Most of mitochondrial genes are encoded on the heavy (H) strand, except for eight tRNAs and ND6. Unusual start codons were identified in COX1 (GTG) and ATP6 (TTG). Six genes (ND2, COX2, COX3, ND3, ND4, and CytB) were terminated by an incomplete stop codon (TA-/T-). A phylogenetic study showed that formed a clade distinct from other species in the subfamily Gobiinae. was most closely related to with 87.04% sequence identity among the four species in the genus .
我们应用下一代测序（NGS）方法构建了……的完整线粒体基因组。所获得的……线粒体基因组（16,590 bp）呈现出典型的结构，包含13个蛋白质编码基因（PCGs）、22个转运RNA（tRNAs）、两个核糖体RNA（rRNAs）和一个控制区（D-loop）。除了8个tRNAs和ND6外，大多数线粒体基因在重链（H）上编码。在COX1（GTG）和ATP6（TTG）中鉴定出异常起始密码子。6个基因（ND2、COX2、COX3、ND3、ND4和CytB）以不完全终止密码子（TA-/T-）终止。系统发育研究表明，……在虾虎鱼亚科中形成了一个与其他物种不同的分支。在……属的四个物种中，……与……关系最为密切，序列同一性为87.04%。
