Klimczak-Bitner Anna Agnieszka, Bitner Jan, Hiruta Komei, Szemraj Janusz
Department of Biomedical Chemistry, Medical University of Lodz, 92-215, Lodz, Poland.
Department of Medicinal Biochemistry, Medical University of Lodz, 92-215, Lodz, Poland.
Biochem Biophys Rep. 2021 Oct 5;28:101147. doi: 10.1016/j.bbrep.2021.101147. eCollection 2021 Dec.
The goal of this research was to analyze the -675 4G/5G (rs1799889) and T-1702A (rs2297864) polymorphisms in esophageal cancer among polish patients, classified as part of the Caucasian population. The analysis of polymorphic gene variants was performed on 35 randomly selected samples excised from patients with esophageal cancer. The tissue specimens were stored as Formalin-Fixed, Paraffin-Embedded (FFPE) blocks. All patients in the sample group were of Caucasian ethnicity. The genotype distribution of T-1702A and -675 polymorphisms was analyzed using the Restriction Fragment Length Polymorphism (RFLP) method. A correlation between the expression of -675 polymorphic form of and alcohol abuse has been found. Additionally, a correlation between the -675 polymorphism and the subtype of EC developed by the patient has been shown. To the best of the authors' knowledge, this is the first report investigating the -675 4G/5G (rs1799889) polymorphism as a potential candidate for a prognostic biomarker of esophageal cancer.
本研究的目的是分析波兰患者（属于高加索人群）食管癌中的-675 4G/5G（rs1799889）和T-1702A（rs2297864）多态性。对从食管癌患者中随机选取的35个样本进行多态性基因变异分析。组织标本以福尔马林固定、石蜡包埋（FFPE）块的形式保存。样本组中的所有患者均为白种人。采用限制性片段长度多态性（RFLP）方法分析T-1702A和-675多态性的基因型分布。已发现与酒精滥用相关的-675多态形式的表达之间存在相关性。此外，还显示了-675多态性与患者所患食管癌亚型之间的相关性。据作者所知，这是首篇研究-675 4G/5G（rs1799889）多态性作为食管癌预后生物标志物潜在候选物的报告。
