Chen Yin, Mao Wenjun, Yang Yupin, Teng Xiancun, Zhu Weiming, Qi Xiaohui, Chen Yanli, Zhao Chunqi, Hou Yujiao, Wang Chunyan, Li Na
Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
Carbohydr Polym. 2012 Jan 4;87(1):218-226. doi: 10.1016/j.carbpol.2011.07.042. Epub 2011 Jul 30.
An extracellular polysaccharide AVP was isolated from the fermented broth of coral-associated fungus Aspergillus versicolor LCJ-5-4. AVP was a mannoglucan with molecular weight of about 7kDa, and the molar ratio of glucose and mannose was 1.7:1.0. On the basis of detailed one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopic analyses, the backbone of AVP was characterized to be composed of (1→6)-linked α-d-glucopyranose and (1→2)-linked α-d-mannopyranose units. The mannopyranose residues in the backbone were substituted mainly at C-6 by the side chain of (1→2)-linked α-d-mannopyranose trisaccharides units. The antioxidant activity of AVP was evaluated with the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals in vitro, and the results indicated that AVP had good antioxidant activity, especially scavenging ability on superoxide radicals. AVP was a novel extracellular polysaccharide with different structural characteristics from other extracellular polysaccharides and could be a potential source of antioxidant.
从与珊瑚相关的真菌杂色曲霉LCJ-5-4的发酵液中分离出一种细胞外多糖AVP。AVP是一种分子量约为7kDa的甘露葡聚糖，葡萄糖与甘露糖的摩尔比为1.7:1.0。基于详细的一维和二维核磁共振（1D和2D NMR）光谱分析，AVP的主链被表征为由（1→6）连接的α-d-吡喃葡萄糖和（1→2）连接的α-d-吡喃甘露糖单元组成。主链中的吡喃甘露糖残基主要在C-6位被（1→2）连接的α-d-吡喃甘露糖三糖单元的侧链取代。通过体外对1,1-二苯基-2-苦基肼（DPPH）、超氧阴离子和羟基自由基的清除能力评估了AVP的抗氧化活性，结果表明AVP具有良好的抗氧化活性，尤其是对超氧阴离子自由基的清除能力。AVP是一种具有与其他细胞外多糖不同结构特征的新型细胞外多糖，可能是一种潜在的抗氧化剂来源。
