红树林丝状真菌ANP2胞外多糖的化学表征及抗氧化性能

Chemical characterization and antioxidant properties of exopolysaccharides from mangrove filamentous fungi ANP2.

作者信息

Prathyusha A M V N, Mohana Sheela G, Bramhachari P V

机构信息

Department of Biotechnology, Krishna University, Machilipatnam, AP, 52100, India.

Departmnt of Biotecnology, Vignan University, Vadlamudi, Guntur, AP, 522213, India.

出版信息

Biotechnol Rep (Amst). 2018 Aug 13;19:e00277. doi: 10.1016/j.btre.2018.e00277. eCollection 2018 Sep.

A filamentous fungi strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as yielded EPS (4.9 ± 0.25 g/L) and whole cell biomass (3.5 ± 0.2 g/L) in MSM during early stationary phase. The crude EPS was purified by chromatography of Q-Sepharose and Superdex-75, affording Ms and MF-1 fractions were obtained, respectively. Chemical analyses revealed that EPS is primarily composed of neutral sugars and proteins. FTIR-spectroscopy revealed presence of hydroxyl, carbonyl groups and glycosidic bonds which correspond to typical heteropolysaccharide. GC analysis demonstrated that MF-1 mainly consist of mannose (72.6%) and glucose (27.3%). Viscosity studies exhibited typical non-Newtonian pseudoplastic behaviour with high viscosity. The scavenging ability on hydroxyl radicals, indicate that MF-1-EPS has good antioxidant activity. Current study demonstrated that MF-1 differed from previously characterized sp., and can serve as potential antioxidant.

从克里希纳河口红树林沉积物中筛选并分离出一株产胞外多糖（EPS）能力良好、表观粘度较高的丝状真菌菌株ANP2。根据分子特征，该选定菌株在MSM中稳定期早期产EPS（4.9±0.25 g/L）和全细胞生物量（3.5±0.2 g/L）。粗EPS通过Q-琼脂糖和Superdex-75色谱法纯化，分别得到Ms和MF-1组分。化学分析表明，EPS主要由中性糖和蛋白质组成。傅里叶变换红外光谱显示存在羟基、羰基和糖苷键，这与典型的杂多糖相对应。气相色谱分析表明，MF-1主要由甘露糖（72.6%）和葡萄糖（27.3%）组成。粘度研究显示出典型的非牛顿假塑性行为且粘度较高。对羟基自由基的清除能力表明，MF-1-EPS具有良好的抗氧化活性。当前研究表明，MF-1与先前表征的菌种不同，可作为潜在的抗氧化剂。