Chen Wei, Wang Wei-Ping, Zhang Hua-Shan, Huang Qin
College of Bioengineering, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
College of Bioengineering, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
Carbohydr Polym. 2012 Jan 4;87(1):614-619. doi: 10.1016/j.carbpol.2011.08.029. Epub 2011 Aug 19.
An ultrasound-assisted procedure for the extraction of water-soluble polysaccharides from the submerged-cultured mycelia of Boletus edulis was investigated using response surface methodology (RSM). Three independent variables were ratio of dried mycelia to water (X: 1:40-1:60), extraction time (X: 6-10min), and ultrasonic temperature (X: 50-70°C). The statistical analysis indicated the independent variables (X, X), the quadratic terms (X and X) and the interaction between X and X had significant effects on the yield of polysaccharides (p<.05). The optimized conditions were 56°C, 1:55 of ratio of dried mycelia to water, and a time of contact of 8.4min. Under these conditions, the experimental yield of polysaccharides was 15.48%, which was well matched with the predictive yield of 15.53%.
采用响应面法（RSM）研究了一种超声辅助从美味牛肝菌深层培养菌丝体中提取水溶性多糖的方法。三个独立变量分别为干菌丝体与水的比例（X₁：1:40 - 1:60）、提取时间（X₂：6 - 10分钟）和超声温度（X₃：50 - 70°C）。统计分析表明，独立变量（X₁、X₂）、二次项（X₁²和X₂²）以及X₁和X₂之间的相互作用对多糖产率有显著影响（p <.05）。优化条件为56°C、干菌丝体与水的比例为1:55以及接触时间为8.4分钟。在这些条件下，多糖的实验产率为15.48%，与预测产率15.53%非常匹配。
