School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan University of Chinese Medicine, Kunming 650500, China.
Yunnan Key Laboratory for Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming 650500, China.
Molecules. 2022 Feb 22;27(5):1463. doi: 10.3390/molecules27051463.
Collett & Hemsl is one of the famous traditional Chinese herbs with satisfactory therapeutic effects on invigorating Qi, nourishing Yin and moistening lungs, in which steroidal saponins are one class of important active substances. The main purpose is to determine the optimal extraction technology of steroidal saponins and evaluate the quality of planted in five different areas. The optimal ultrasonic-assisted extraction (UAE) technology was established by using single-factor experiments and the response surface methodology (RSM), and the determination method of high-performance liquid chromatography (HPLC) for dioscin and diosgenin, two primary types of acid-hydrolyzed steroidal saponins, was constructed with good linear range and precision. The results showed that UAE was an efficient extraction method for steroidal saponins, and the extraction yield was significantly affected by the liquid-solid ratio. The optimal extraction technology was generated following a liquid-solid ratio of 10:1 (mL/g), an ethanol concentration of 85% (/), an extraction time of 75 min, an extraction temperature of 50 °C and three extractions, of which these parameters were in line with the predicted values calculated by RSM. Considering only dioscin and diosgenin, the quality of planted at five sample plots presented non-significant difference. However, the content of diosgenin in Pingbian Prefecture (PB) was higher than that of the other four areas with a value of 0.46 mg/g. Taken together, the optimal UAE technology for steroidal saponins was determined via RSM. The quality evaluation revealed that there was a non-significant difference among planted in different areas based on the contents of the sum of dioscin and diosgenin. This work has important reference value for the exploitation and utilization of
Collett & Hemsl 是一种著名的中草药，对补气、滋阴润肺有满意的疗效，其中甾体皂苷是一类重要的活性物质。本研究的主要目的是确定甾体皂苷的最佳提取工艺，并评价五个不同产地种植的质量。采用单因素实验和响应面法（RSM）建立了最佳超声辅助提取（UAE）工艺，并采用高效液相色谱（HPLC）法建立了两种主要类型的酸水解甾体皂苷——薯蓣皂苷元和薯蓣皂苷的测定方法，具有良好的线性范围和精密度。结果表明，UAE 是一种有效的提取甾体皂苷的方法，提取收率受液固比的显著影响。最佳提取工艺为液固比 10:1（mL/g）、乙醇浓度 85%（/）、提取时间 75 min、提取温度 50°C、提取 3 次，这些参数与 RSM 计算的预测值相符。仅考虑薯蓣皂苷元和薯蓣皂苷，五个采样点种植的质量没有显著差异。然而，平边县（PB）的薯蓣皂苷元含量高于其他四个地区，为 0.46mg/g。综上所述，通过 RSM 确定了 Collett & Hemsl 甾体皂苷的最佳 UAE 工艺。质量评价表明，基于薯蓣皂苷元和薯蓣皂苷含量，不同产地种植的质量无显著差异。这项工作对该植物的开发利用具有重要的参考价值。
