La Celle P L
Blood Cells. 1986;12(1):179-89.
The effects of leukocytes on blood rheology in the microcirculation may be predicted to result from the rheological characteristics of the individual white blood cell types and their behavior at entrances of small vessels; their influence on flow resistance of blood due to their disturbance of erythrocyte flow; and the obstruction caused by their adherence to endothelial cells or geometrical hindrance of their flow at irregular or narrow regions. Deformation of leukocytes in micropipettes indicates the relative rigidity of lymphocytes and blast cells from leukemias in contrast to granulocyte viscoelastic properties, and entry times for lymphocytes in 2.6-4.6-microns channels were 11-151 s but milliseconds in 9.1-micron tubes. Lymphocytes and erythrocytes rarely (less than 1%) adhered to cultured endothelium; however, granulocytes adhered avidly at shear stresses of 10-100 dyn/cm2, typical of microcirculation. In the 9.1-microns flow tube at Hct = 17.7 +/- 2.6%, increasing [WBC] caused a linear increase in flow resistance, but above [WBC] = 1000/mm3 resistance tended to plateau. These data support the interpretation that granulocyte adherence to endothelial cells with the potential for obstruction of flow may be a more significant rheological consequence of leukocytes in blood than their resistance to flow because of their relative rigidity or their influence on flow resistance by perturbation of the erythrocyte flow.
白细胞对微循环中血液流变学的影响可能源于单个白细胞类型的流变学特性及其在小血管入口处的行为；它们因干扰红细胞流动而对血液流动阻力产生的影响；以及它们黏附于内皮细胞所造成的阻塞或在不规则或狭窄区域对其流动形成的几何阻碍。微量移液器中白细胞的变形表明，与粒细胞的黏弹性特性相比，白血病淋巴细胞和母细胞的相对刚性，淋巴细胞在2.6 - 4.6微米通道中的进入时间为11 - 151秒，但在9.1微米管中为毫秒级。淋巴细胞和红细胞很少（少于1%）黏附于培养的内皮细胞；然而，粒细胞在10 - 100达因/平方厘米的剪切应力下会强烈黏附，这是微循环的典型剪切应力。在血细胞比容为17.7 +/- 2.6%的9.1微米流动管中，白细胞浓度升高会导致流动阻力呈线性增加，但白细胞浓度高于1000/立方毫米时，阻力趋于平稳。这些数据支持这样一种解释，即粒细胞黏附于内皮细胞并有可能阻塞血流，这可能是血液中白细胞比因其相对刚性或因干扰红细胞流动而对血流阻力产生的影响更为重要的流变学后果。
