Tantanate Chaicharoen, Talabthong Supavat, Lamyai Phenluck
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkoknoi, Bangkok, Thailand.
Lab Med. 2022 May 5;53(3):e69-e73. doi: 10.1093/labmed/lmab090.
To indicate the ability to disaggregate platelet clumps by vortex mixing and kanamycin supplementation in EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) specimens.
For patients with EDTA-PTCP, citrate-anticoagulated, primary EDTA-anticoagulated, vortex-mixed, and kanamycin-treated specimens were tested for complete blood count and platelet-related parameters.
Forty-eight specimens were included. Nineteen (39.6%) of the vortex-mixed specimens and 42 (87.5%) of the kanamycin-treated specimens revealed platelet counts more than those of the primary EDTA specimens, with levels exceeding 100 × 109/L. The platelet count and platelet recovery of the kanamycin-treated specimens were higher than those of the vortex-mixed specimens.
Kanamycin supplementation to EDTA-PTCP blood may be considered as an alternative approach when the recollection of specimens is impractical. Only platelet-related parameters from kanamycin treatment were suitable for guiding patient management. Further studies about the impact of these methods in patients with various conditions, such as in patients with advanced kidney disease, should be conducted.
表明在乙二胺四乙酸（EDTA）依赖性假性血小板减少症（EDTA-PTCP）标本中，通过涡旋混合和添加卡那霉素来分解血小板凝块的能力。
对于EDTA-PTCP患者，对枸橼酸盐抗凝、初次EDTA抗凝、涡旋混合及卡那霉素处理的标本进行全血细胞计数和血小板相关参数检测。
纳入48份标本。涡旋混合标本中有19份（39.6%），卡那霉素处理标本中有42份（87.5%）的血小板计数高于初次EDTA标本，且水平超过100×10⁹/L。卡那霉素处理标本的血小板计数和血小板回收率高于涡旋混合标本。
当再次采集标本不切实际时，可考虑在EDTA-PTCP血液中添加卡那霉素作为一种替代方法。只有卡那霉素处理后的血小板相关参数适合指导患者管理。应进一步研究这些方法对各种情况患者的影响，如晚期肾病患者。
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