对复合耐药结核分枝杆菌的贝达喹啉活性。

activity of bedaquiline against complex.

机构信息

Moscow Research and Clinical Center for Tuberculosis Control of the Moscow Government Health Department (MRCCTC), 107114 Moscow, Russia.

National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia, 107114 Moscow, Russia.

出版信息

J Med Microbiol. 2021 Oct;70(10). doi: 10.1099/jmm.0.001439.

DOI:10.1099/jmm.0.001439

PMID:34668850

Abstract

Nontuberculous mycobacteria (NTM) are widespread in the environment and can cause various diseases in humans, especially immunocompromised patients. Treatment of diseases caused by NTM is a complicated issue, mainly due to the resistance of the pathogen to most antimicrobial agents. Bedaquiline (Bdq) is now widely used for the treatment of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB). The main goal of our study was to evaluate the activity of Bdq against complex (MAC), the most common species among NTM. A total of 166 MAC cultures (124 and 42 ) were studied. The minimum inhibitory concentrations (MICs) of Bdq for and were obtained by twofold serial dilutions in the Middlebrook 7H9 medium. MIC ranges were determined and the MIC, MIC and ECOFF values were obtained. The MICs in respect of ranged from 0.003 to 1.0 µg ml; those for ranged from 0.003 to 0.5 µg ml. The Bdq MIC and MIC values were found to be 0.015 and 0.12 µg ml respectively, for and 0.007 and 0.06 µg ml, respectively, for . The tentative ECOFF values for and were 0.12 and 0.06 µg ml, respectively. The main bedaquiline susceptibility parameters for MAC strains isolated in the Moscow region were determined.

摘要

非结核分枝杆菌（NTM）广泛存在于环境中，可导致人类罹患各种疾病，尤其是免疫功能低下的患者。NTM 引起的疾病的治疗是一个复杂的问题，主要是由于病原体对大多数抗菌药物的耐药性。贝达喹啉（Bdq）目前广泛用于治疗耐多药（MDR）和广泛耐药（XDR）结核病（TB）。我们研究的主要目的是评估 Bdq 对复合分枝杆菌（MAC）的活性，MAC 是 NTM 中最常见的物种。共研究了 166 株 MAC 培养物（124 株和 42 株）。通过在 Middlebrook 7H9 培养基中进行两倍连续稀释来获得 Bdq 对和的最小抑菌浓度（MIC）。确定 MIC 范围，并获得 MIC、MIC 和 ECOFF 值。对的 MIC 范围为 0.003 至 1.0 µg ml；对的 MIC 范围为 0.003 至 0.5 µg ml。Bdq 的 MIC 和 MIC 值分别为 0.015 和 0.12 µg ml ，为 0.007 和 0.06 µg ml，为。和的暂定 ECOFF 值分别为 0.12 和 0.06 µg ml。确定了莫斯科地区分离的 MAC 菌株的主要贝达喹啉药敏参数。