Department of Molecular Biology, Faculty of Medical Science in Katowice, Medical University of Silesia, Katowice, Poland.
Department of Medical Genetics, Faculty of Medical Science in Katowice, Medical University of Silesia, Katowice, Poland.
Acta Biochim Pol. 2021 Oct 20;68(4):745-750. doi: 10.18388/abp.2020_5645.
Abdominal aortic aneurysm is a process involving the disruption and reconstruction of the extracellular matrix and the apoptosis of smooth muscle cells under the strong influence of the immune system. Thrombospondins are proteins that influence a wide range of cell-matrix interactions. While THBS1 and THBS2 are widely studied, the effects of THBS3 on extracellular matrix and vascular cells are poorly understood. Additionally, it is not known whether expression of these genes' changes along the aneurysm tissue. Here we analyzed the expression of THBSs mRNA isolated from the harvested tissues along the aneurysm divided into three zones based on their morphology. Total mRNA was isolated from 13 male patients undergoing scheduled open aortic repair, with each aneurysm divided into a proximal part, an aneurysm bag, and a distal part with border tissue as a control. Two step real-time PCR analysis with random hexamers was performed, which allowed the detection of significantly increased expression of all analyzed thrombospondins, especially THBS3, at the control tissue. Overexpression of THBSs may have a destabilizing effect on the structure of the extracellular matrix by affecting both the matrix producing cells and by inhibiting the activity of matrix proteins.
腹主动脉瘤是一个涉及细胞外基质的破坏和重建以及平滑肌细胞凋亡的过程，免疫系统在这个过程中起着重要作用。血栓反应蛋白是一类影响广泛的细胞基质相互作用的蛋白。虽然 THBS1 和 THBS2 已经得到了广泛的研究，但 THBS3 对细胞外基质和血管细胞的影响还知之甚少。此外，这些基因的表达是否随着动脉瘤组织的变化而变化也尚不清楚。在这里，我们分析了从根据形态学分为三个区域的收集的动脉瘤组织中分离的 THBSs mRNA 的表达。从 13 名男性患者中分离总 mRNA ，他们正在接受预定的开放主动脉修复，每个动脉瘤分为近端部分、动脉瘤袋和有边界组织的远端部分作为对照。使用随机六聚体进行两步实时 PCR 分析，允许检测到所有分析的血栓反应蛋白的表达显著增加，尤其是在对照组织中 THBS3 的表达显著增加。THBSs 的过表达可能通过影响基质产生细胞和抑制基质蛋白的活性对细胞外基质的结构产生不稳定作用。
