Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, Harbin, 150080, China.
Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, Harbin, 150080, China; National Sugar Crops Improvement Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, 150080, China.
Plant Physiol Biochem. 2021 Nov;168:294-304. doi: 10.1016/j.plaphy.2021.10.017. Epub 2021 Oct 14.
Sugar beet (Beta vulgaris L.) has a high demand for B, and B deficiency inhibits normal growth and productivity. However, there is a lack of information on how B deficiency affects the growth of beet at the transcriptome level, and the factors that govern B utilisation efficiency. This study aimed to identify the genes differentially expressed under B deficiency and those that underlie the mechanisms of efficient B use in two sugar beet cultivars. Accordingly, B-efficient (H, KWS1197) and B-inefficient (L, KWS0143) sugar beet cultivars were used, and two levels of boron were employed in the hydroponic experiments: B0.1 (0.1 μM B, deficiency) and B50 (50 μM B, CK). The results showed that B deficiency inhibited leaf growth, significantly reduced B concentration and B transfer coefficient, and increased peroxidase (POD) activity and malondialdehyde and proline content. The transcriptome data showed that the B-efficient variety exhibited more differentially expressed genes than the B-inefficient variety. Metabolic pathways were the most critical pathways involved in the B deficiency response. The expression of POD, bHLH, WRKY transcription factors, and nodulin26-like intrinsic protein (NIP5;1) were upregulated in the KWS1197 variety. In conclusion, the KWS1197 variety had physiological advantages and a highly efficient B utilisation molecular mechanism, contributing to a high B deficiency tolerance. This study provides a theoretical basis for the adaptation mechanism to B deficiency in sugar beets.
糖甜菜（Beta vulgaris L.）对 B 有很高的需求，B 缺乏会抑制其正常生长和生产力。然而，关于 B 缺乏如何影响甜菜在转录组水平上的生长以及控制 B 利用效率的因素，我们知之甚少。本研究旨在鉴定在 B 缺乏下差异表达的基因，以及在两个糖甜菜品种中高效利用 B 的机制基础上的基因。因此，使用了 B 高效（H，KWS1197）和 B 低效（L，KWS0143）的糖甜菜品种，并在水培实验中使用了两个硼水平：B0.1（0.1 μM B，缺乏）和 B50（50 μM B，CK）。结果表明，B 缺乏抑制叶片生长，显著降低 B 浓度和 B 转移系数，增加过氧化物酶（POD）活性以及丙二醛和脯氨酸含量。转录组数据表明，B 高效品种比 B 低效品种表现出更多差异表达的基因。代谢途径是参与 B 缺乏反应的最关键途径。在 KWS1197 品种中，POD、bHLH、WRKY 转录因子和类根瘤蛋白 26 样内在蛋白（NIP5;1）的表达上调。总之，KWS1197 品种具有生理优势和高效利用 B 的分子机制，有助于提高对 B 缺乏的耐受性。本研究为糖甜菜适应 B 缺乏的机制提供了理论依据。
