School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
School of Astronautics, Harbin Institute of Technology, Harbin 150001, China.
Int J Mol Sci. 2024 Aug 18;25(16):8989. doi: 10.3390/ijms25168989.
Sugar beet is a significant sugar crop in China, primarily cultivated in arid regions of the north. However, drought often affects sugar beet cultivation, leading to reduced yield and quality. Therefore, understanding the impact of drought on sugar beets and studying their drought tolerance is crucial. Previous research has examined the role of SPL ( promoter-binding protein-like) transcription factors in plant stress response; however, the precise contribution of SPLs to the drought stress response in sugar beets has yet to be elucidated. In this study, we identified and examined the , , and genes in sugar beets, investigating their performance during the seedling stage under drought stress. We explored their drought resistance characteristics using bioinformatics, quantitative analysis, physiological experiments, and molecular biology experiments. Drought stress and rehydration treatments were applied to sugar beet seedlings, and the expression levels of , , and genes in leaves were quantitatively analyzed at 11 different time points to evaluate sugar beets' response and tolerance to drought stress. Results indicated that the expression level of the genes in leaves was upregulated during the mid-stage of drought stress and downregulated during the early and late stages. Additionally, the expression level of the gene gradually increased with the duration of drought stress. Through analyzing changes in physiological indicators during different time periods of drought stress and rehydration treatment, we speculated that the regulation of genes is associated with sugar beet drought resistance and their participation in drought stress response. Furthermore, we cloned the CDS sequences of , , and genes from sugar beets and conducted sequence alignment with the database to validate the results. Subsequently, we constructed overexpression vectors, named 35S::, 35S::, and 35S::, and introduced them into sugar beets using -mediated methods. Real-time fluorescence quantitative analysis revealed that the expression levels of /7/ genes in transgenic sugar beets increased by 40% to 80%. The drought resistance of transgenic sugar beets was significantly enhanced compared with the control group.
甜菜是中国重要的糖料作物，主要种植在北方干旱地区。然而，干旱常常影响甜菜的种植，导致产量和品质下降。因此，了解干旱对甜菜的影响，研究其抗旱性至关重要。先前的研究已经研究了 SPL（启动子结合蛋白样）转录因子在植物胁迫反应中的作用；然而，SPLs 对甜菜干旱胁迫反应的确切贡献尚未阐明。在这项研究中，我们鉴定并研究了甜菜中的 、 、 基因，研究了它们在干旱胁迫下幼苗期的表现。我们使用生物信息学、定量分析、生理实验和分子生物学实验来探索它们的抗旱特性。对甜菜幼苗进行干旱胁迫和复水处理，在 11 个不同时间点定量分析叶片中 、 、 基因的表达水平，以评估甜菜对干旱胁迫的响应和耐受能力。结果表明，干旱胁迫中期叶片中 基因的表达水平上调，而在干旱胁迫的早期和晚期下调。此外， 基因的表达水平随着干旱胁迫持续时间的增加而逐渐增加。通过分析不同干旱胁迫和复水处理时期生理指标的变化，我们推测 基因的调控与甜菜抗旱性有关，它们参与了干旱胁迫反应。此外，我们从甜菜中克隆了 、 、 基因的 CDS 序列，并与数据库进行序列比对以验证结果。随后，我们构建了过表达载体，命名为 35S:: 、 35S:: 、 35S:: ，并通过 -介导的方法将其导入甜菜中。实时荧光定量分析显示，转基因甜菜中 /7/基因的表达水平增加了 40%至 80%。与对照组相比，转基因甜菜的抗旱性显著增强。
