sp. nov., novel actinobacteria isolated from pine forest soil in Poland.

The taxonomic status of two filamentous actinobacteria, isolates NF23 and NL8, recovered from the litter layer of a pine forest soil in Poland was established in a genome-based polyphasic study. The isolates showed a combination of chemotaxonomic, morphological and physiological properties associated with their classification in the genus . They formed a well supported lineage within the 16S rRNA gene tree and were most closely related to the type strains of (99.1%), (99.9 %) and (99.1 %), and like them, were found to have large genomes (10.8 and 11.5 Mbp, respectively). A phylogenomic tree based on the draft genomes of isolates NF23 and NL8 and their phylogenetic neighbours showed that they formed a distinct branch in the clade that was most closely related to DSM 111109. The isolates shared a combination of genomic, genotypic and phenotypic features, and had high average nucleotide index (ANI) and digital DNA:DNA hybridization (dDDH) similarities consistent with their assignment to the same species. The isolates were distinguished from the and strains by a wealth of taxonomic data and by low ANI (84.9-93.9 %) and dDDH (29.6-54.7 %) values. It is proposed that the isolates be classified in the genus as sp. nov. with isolate NL8 (=DSM 111110=PCM 3045) as the type strain. The genomes of strains NF23 and NL8 are rich in natural product-biosynthetic gene clusters hence these strains have the potential to synthesize new specialised metabolites.