Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87 100 Toruń, Poland.
School of Natural and Environmental Sciences, Ridley Building 2, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom.
Syst Appl Microbiol. 2021 Jan;44(1):126164. doi: 10.1016/j.syapm.2020.126164. Epub 2020 Dec 8.
Two actinobacteria, strains NF3 and NH11, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora. They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C and anteiso-C as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928 and Catenulispora rubra DSM 44948 sharing sequence similarities with the latter of 98.8 and 99.0%, respectively. The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%. The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree. Isolates NF3 and NH11 belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities. They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species. Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp. nov., the type strain is NH11 (=DSM 111109 =PCM 3046). An emended description is given for C. rubra based on data acquired in the present study. Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites. In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
两株放线菌，NF3 和 NH11 菌株，从波兰托伦附近松林土壤中分离得到，它们的多种化学分类学和形态学特性被鉴定为链霉菌属的 Catenulispora 属。它们产生广泛分枝的稳定菌丝体，肽聚糖中的二氨基庚二酸为 LL-二氨基庚二酸，全细胞糖为阿拉伯糖，四氢、六氢和八氢甲基萘醌，具有九个异戊二烯单元作为主要异戊二烯醇，异-C 和 anteiso-C 作为主要脂肪酸，并与 Catenulispora acidiphila DSM 44928 和 Catenulispora rubra DSM 44948 一起在 Catenulispora 16S rRNA 基因树中形成一个支持良好的分支，与后者的序列相似性分别为 98.8%和 99.0%。为分离株和 C. rubra 菌株生成的全基因组序列大小范围为 11.20 到 12.80 Mbp，相应的 DNA G+C 值为 69.9-70.0%。分离株和 C. acidiphila 和 C. rubra 菌株在放线菌系统发育基因组树中形成一个支持良好的分支。分离株 NF3 和 NH11 属于同一物种，因为它们具有相同的 16S rRNA 基因序列，具有许多化学分类学、培养和表型特征，并且显示出非常高的平均核苷酸同一性（ANI）和数字 DNA：DNA 同源性（dDDH）相似性。它们可以通过化学分类学和表型特性的组合以及低于用于将密切相关的菌株分配到不同物种的这些指标的 ANI 和 dDDH 值来与最接近的系统发育邻居区分开来。因此，我们建议将分离株分类为一种新的链霉菌属，即 Catenulispora pinisilvae sp. nov.，其模式菌株为 NH11（=DSM 111109 =PCM 3046）。根据本研究获得的数据，对 C. rubra 进行了修订描述。对分离株和 C. acidiphila 和 C. rubra 菌株的基因组草案进行分析表明，存在许多具有合成新型药物样代谢物潜力的生物合成基因簇。体外筛选表明，分离株抑制革兰氏阳性菌和属于镰刀菌属的小麦病原体的生长。
