The Jackson Laboratory, Bar Harbor, ME, USA.
The Hospital for Sick Children and the Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; The Gairdner Foundation, Toronto, ON, Canada.
Cell Stem Cell. 2021 Nov 4;28(11):1896-1906. doi: 10.1016/j.stem.2021.10.001. Epub 2021 Oct 20.
Pluripotent cells in the mammalian embryo undergo state transitions marked by changes in patterns of gene expression and developmental potential as they progress from pre-implantation through post-implantation stages of development. Recent studies of cultured mouse and human pluripotent stem cells (hPSCs) have identified cells representative of an intermediate stage (referred to as the formative state) between naive pluripotency (equivalent to pre-implantation epiblast) and primed pluripotency (equivalent to late post-implantation epiblast). We examine these recent findings in light of our knowledge of peri-implantation mouse and human development, and we consider the implications of this work for deriving human embryo models from pluripotent cells.
哺乳动物胚胎中的多能细胞在从着床前到着床后发育阶段的过程中，经历了基因表达模式和发育潜能变化的状态转变。最近对培养的小鼠和人类多能干细胞（hPSCs）的研究鉴定了具有中间状态的细胞（称为形成状态），该状态处于原始多能性（相当于着床前外胚层）和初始多能性（相当于晚期着床后外胚层）之间。我们根据对植入前小鼠和人类发育的了解来研究这些最近的发现，并考虑这项工作对于从多能细胞中获得人类胚胎模型的意义。
