微藻-细菌协同作用增强土霉素的生物降解。
Enhanced biodegradation of chlortetracycline via a microalgae-bacteria consortium.
机构信息
School of Materials and Environmental Engineering, Yantai University, Yantai, China.
Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
出版信息
Bioresour Technol. 2022 Jan;343:126149. doi: 10.1016/j.biortech.2021.126149. Epub 2021 Oct 18.
Microbial removal of Chlortetracycline (CTC) at low CTC concentrations (in the order of 10-20 mg/L) has been reported. In this study, a novel microalgae-bacteria consortium was developed for effective CTC biodegradation at higher concentrations (up to 80 mg/L). The microalgae-bacteria consortium is resistant to up to 80 mg/L CTC, while the pure microalgal culture could only tolerate 60 mg/L CTC. CTC removal in the initial 12 h was primarily via biosorption by the microalgae-bacteria consortium and the adsorption capacity increased from 61.71 to 102.53 mg/g biomass in 12 h. Further, CTC biodegradation by the microalgae-bacteria consortium was catalyzed by extracellular enzymes secreted under antibiotic stress. The symbiotic bacterial diversity was analyzed by high throughput sequencing. The aerobic bacteria Porphyrobacter and Devosia were the dominant genera in the consortium. In the presence of CTC, a microbial community shift occurred with Chloroptast, Spingopyxis, and Brevundimonas being the dominant genera.
已报道过微生物在低浓度（约 10-20mg/L）下去除金霉素（CTC）的情况。在本研究中，开发了一种新型的微藻-细菌共生体，可在更高浓度（高达 80mg/L）下有效进行 CTC 生物降解。微藻-细菌共生体可耐受高达 80mg/L 的 CTC，而纯微藻培养物只能耐受 60mg/L 的 CTC。在最初的 12 小时内，CTC 的去除主要通过微藻-细菌共生体的生物吸附完成，并且在 12 小时内吸附能力从 61.71 增加到 102.53mg/g 生物质。此外，微藻-细菌共生体通过在抗生素胁迫下分泌的胞外酶催化 CTC 生物降解。通过高通量测序分析了共生细菌的多样性。好氧菌 Porphyrobacter 和 Devosia 是共生体中的主要属。在 CTC 存在的情况下，微生物群落发生了变化，Chloroptast、Spingopyxis 和 Brevundimonas 成为主要属。
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