Huang Ruohan, Liu Wan, Su Jinghua, Li Shihao, Wang Liqing, Jeppesen Erik, Zhang Wei
Key laboratory of Exploration and Utilization of Aquatic Genetic Resources of the Ministry of Education, Engineering Research Center of Environmental DNA and Ecological Water Health Assessment, Shanghai Ocean University, Shanghai, China.
Research Institute of Natural Ecology Conservation, Shanghai Academy of Environmental Sciences, Shanghai, China.
Front Plant Sci. 2023 Jul 5;14:1193668. doi: 10.3389/fpls.2023.1193668. eCollection 2023.
In recent years, antibiotics pollution has caused serious harm to the aquatic environment, and microalgae mediated degradation of antibiotics has attracted increasing attention. However, the potential toxicity of antibiotics to keystone microalgae species or their microalgae consortia, and the impact of microalgal diversity on antibiotic removal need to be further studied. In this study, we investigated the removal efficiency and tolerance of five freshwater microalgae (, , sp., , and ) and their microalgae consortia to sulfamethoxazole (SMX). We found that the removal efficiency of SMX by reached 49%, while the other four microalgae ranged between 9% and 16%. In addition, , S. , and sp. had better tolerance to SMX than , and their growth and photosynthesis were less affected. At 10 and 50 mg/L SMX, the removal capacity of SMX by mixed microalgae consortia was lower than that of except for the consortium with and . The consortia generally showed higher sensitivity towards SMX than the individual species, and the biochemical characteristics (photosynthetic pigment, chlorophyll fluorescence parameters, superoxide anion (O ), superoxide dismutase activity (SOD), malondialdehyde (MDA) and extracellular enzymes) were significantly influenced by SMX stress. Therefore, the removal of antibiotics by microalgae consortia did not increase with the number of microalgae species. Our study provides a new perspective for the selection of microalgal consortia to degrade antibiotics.
近年来，抗生素污染已对水环境造成严重危害，微藻介导的抗生素降解受到越来越多的关注。然而，抗生素对关键微藻物种或其微藻群落的潜在毒性，以及微藻多样性对抗生素去除的影响仍需进一步研究。在本研究中，我们调查了五种淡水微藻（、、 种、、和）及其微藻群落对磺胺甲恶唑（SMX）的去除效率和耐受性。我们发现，对SMX的去除效率达到49%，而其他四种微藻的去除效率在9%至16%之间。此外，、和 种对SMX的耐受性优于，其生长和光合作用受影响较小。在10和50 mg/L SMX浓度下，除了与 和 组成的群落外，混合微藻群落对SMX的去除能力低于。群落对SMX的敏感性通常高于单个物种，并且生化特性（光合色素、叶绿素荧光参数、超氧阴离子（O）、超氧化物歧化酶活性（SOD）、丙二醛（MDA）和胞外酶）受到SMX胁迫的显著影响。因此，微藻群落对抗生素的去除能力并不会随着微藻物种数量的增加而提高。我们的研究为选择用于降解抗生素的微藻群落提供了新的视角。
