Fantone Sonia, Tossetta Giovanni, Di Simone Nicoletta, Tersigni Chiara, Scambia Giovanni, Marcheggiani Fabio, Giannubilo Stefano R, Marzioni Daniela
Department of Experimental and Clinical Medicine, Università Politecnica Delle Marche, 60126, Ancona, Italy.
Clinic of Obstetrics and Gynaecology, Department of Clinical Sciences, Università Politecnica Delle Marche, Salesi Hospital, Azienda Ospedaliero Universitaria, Ancona, Italy.
Cell Tissue Res. 2022 Jan;387(1):123-130. doi: 10.1007/s00441-021-03543-3. Epub 2021 Oct 21.
CD93, also known as complement component C1q receptor, is expressed on the surface of different cellular types such as monocytes, neutrophils, platelets, microglia, and endothelial cells, and it plays a pivotal role in cell proliferation, cell migration, and formation of capillary-like structures. These processes are strictly regulated, and many fetal and maternal players are involved during placental development. At present, there are no studies in literature regarding CD93 in placental development, so we investigated CD93 expression in first and third trimester and PE placentas by immunohistochemistry and western blotting analysis. In addition, we performed in vitro experiments under oxidative stress conditions to demonstrate how oxidative stress acts on CD93 protein expression. Our data showed that CD93 was expressed in villous cytotrophoblast cells, in some fetal vessels of first and third trimester and PE placentas and in the extravillous cytotrophoblast of cell columns in the first trimester placentas. Moreover, we detected a significant decrease of CD93 expression in third trimester and PE placentas compared to first trimester placentas, while no differences were detected between third and PE placentas. No differences of CD93 expression were detected in oxidative stress conditions. We suggest that CD93 can guide extravillous cytotrophoblast migration through β1-integrin in uterine spiral arteries during placentation in the first trimester of pregnancy and that the decrease of CD93 expression in third trimester and PE placentas could be linked to the poor extravillous cytotrophoblast cells migration. So, it might be interesting to understand the role of CD93 in the first phases of PE onset.
CD93，也被称为补体成分C1q受体，在不同细胞类型的表面表达，如单核细胞、中性粒细胞、血小板、小胶质细胞和内皮细胞，并且在细胞增殖、细胞迁移以及毛细血管样结构的形成中发挥关键作用。这些过程受到严格调控，在胎盘发育过程中有许多胎儿和母体相关因素参与。目前，文献中尚无关于CD93在胎盘发育方面的研究，因此我们通过免疫组织化学和蛋白质印迹分析研究了CD93在孕早期和孕晚期胎盘以及子痫前期胎盘中的表达。此外，我们在氧化应激条件下进行了体外实验，以证明氧化应激如何作用于CD93蛋白表达。我们的数据显示，CD93在绒毛细胞滋养层细胞、孕早期和孕晚期胎盘以及子痫前期胎盘的一些胎儿血管中以及孕早期胎盘细胞柱的绒毛外细胞滋养层中表达。此外，我们检测到与孕早期胎盘相比，孕晚期和子痫前期胎盘的CD93表达显著降低，而孕晚期和子痫前期胎盘之间未检测到差异。在氧化应激条件下未检测到CD93表达的差异。我们认为，在妊娠早期胎盘形成过程中，CD93可通过β1整合素引导绒毛外细胞滋养层在子宫螺旋动脉中迁移，而孕晚期和子痫前期胎盘中CD93表达的降低可能与绒毛外细胞滋养层细胞迁移不良有关。因此，了解CD93在子痫前期发病早期阶段的作用可能会很有意思。
