利用乙酰丙酸衍生的羟基戊酸进行聚羟基烷酸酯的代谢工程生产。
Metabolic Engineering of for Production of Polyhydroxyalkanoates with Hydroxyvaleric Acid Derived from Levulinic Acid.
机构信息
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
Department of Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
出版信息
J Microbiol Biotechnol. 2022 Jan 28;32(1):110-116. doi: 10.4014/jmb.2108.08016.
Polyhydroxyalkanoates (PHAs) are emerging as alternatives to plastics by replacing fossil fuels with renewable raw substrates. Herein, we present the construction of engineered strains to produce short-chain-length PHAs (scl-PHAs), including the monomers 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) produced from levulinic acid (LA). First, an strain expressing genes () from the LA metabolic pathway of KT2440 was constructed to generate 4HV-CoA and 3HV-CoA. Second, both PhaAB enzymes from H16 were expressed to supply 3-hydroxybutyrate (3HB)-CoA from acetyl-CoA. Finally, PHA synthase (PhaC) from was introduced for the subsequent polymerization of these three monomers. The resulting strains produced four PHAs (w/w% of dry cell weight): 9.1 wt% P(4HV), 1.7 wt% P(3HV--4HV), 24.2 wt% P(3HB--4HV), and 35.6 wt% P(3HB--3HV--4HV).
聚羟基脂肪酸酯（PHAs）正在作为塑料的替代品出现，它们用可再生原料替代化石燃料。在此，我们构建了工程菌株以生产短链长 PHAs（scl-PHAs），包括由乙酰丙酸（LA）生产的单体 4-羟基戊酸（4HV）和 3-羟基戊酸（3HV）。首先，构建了表达来自 KT2440 的 LA 代谢途径基因的工程菌株，以生成 4HV-CoA 和 3HV-CoA。其次，表达来自 H16 的两种 PhaAB 酶，以提供乙酰辅酶 A 合成 3-羟基丁酸（3HB）-CoA。最后，引入来自的 PHA 合酶（PhaC）用于随后聚合这三种单体。所得工程菌株产生了四种 PHAs（占干细胞重量的重量%）：9.1 wt% P（4HV）、1.7 wt% P（3HV-4HV）、24.2 wt% P（3HB-4HV）和 35.6 wt% P（3HB-3HV-4HV）。
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