Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, 16846-13114, Tehran, Iran.
Sci Rep. 2021 Oct 21;11(1):20805. doi: 10.1038/s41598-021-00363-x.
In this work, amine-carbon quantum dots (CQDs)/rhodamine B (RhB) ratiometric fluorescent (RF) sensor was employed for effective and selective determination of tamsulosin hydrochloride (TMS) based on a dual-emission fluorescence system. Although the function of amine-CQDs is to transfer the specific interaction between TMS and sensor into detectable fluorescence (FL) signals, RhB as a reference unit has been employed to omit internal and external effects. The FL signal was quenched by adding the TMS at 442 nm; nevertheless, it did not change at 569 nm. The material characterization and investigation of the sensing mechanism were done. The optimization of pH, the volumetric ratio of CQDs to RhB, and interaction time parameters were carried out by the one-variable-at-a-time (OVAT) method. The quantitative analysis of the concentration of TMS for this RF sensor in a linear range of 0.446-7.083 μg mL (1.091-17.338 μM) was obtained (R = 0.9969, n = 3) under optimum conditions. The limit of detection and quantitation values were estimated to be 0.033 μg mL (0.081 μM) and 0.109 μg mL (0.267 μM), respectively. The repeatability of intra-day and inter-day were less than one percent. This inexpensive RF probe was well applied to determine TMS in biological fluids, and acceptable achievements were obtained.
在这项工作中，采用了胺基-碳量子点（CQDs）/罗丹明 B（RhB）比率荧光（RF）传感器，基于双发射荧光系统，有效地、选择性地测定了盐酸坦索罗辛（TMS）。虽然胺基-CQDs 的作用是将 TMS 与传感器之间的特定相互作用转化为可检测的荧光（FL）信号，但 RhB 作为参比单元已被用于省略内部和外部影响。FL 信号在添加 TMS 时在 442nm 处被猝灭，但在 569nm 处没有变化。进行了材料表征和传感机制的研究。通过单变量法（OVAT）对 pH 值、CQDs 与 RhB 的体积比和相互作用时间参数进行了优化。在最佳条件下，该 RF 传感器对 TMS 浓度在 0.446-7.083μgmL（1.091-17.338μM）的线性范围内进行了定量分析（R=0.9969，n=3）。检测限和定量值分别估计为 0.033μgmL（0.081μM）和 0.109μgmL（0.267μM）。日内和日间重复性小于 1%。这种廉价的 RF 探针很好地应用于生物液中 TMS 的测定，并取得了可接受的结果。
