Zheng Yahong, Ding Yuting, Xu Mengran, Chen Haoran, Zhang Hui, Liu Yanyan, Shen Weihua, Li Jiabin
Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
Anhui Center for Surveillance of Bacterial Resistance, Hefei, Anhui, People's Republic of China.
J Inflamm Res. 2021 Oct 9;14:5215-5225. doi: 10.2147/JIR.S334581. eCollection 2021.
-induced liver abscess (KLA) is a type of pyogenic liver abscess (PLA), which is a distinct invasive syndrome that has been increasingly reported worldwide over the past two decades. The intestinal microbiota is increasingly recognized as an important modulator that can promote and maintain host immune homeostasis. However, its precise role in liver abscess is unknown. We aimed to investigate the function of the gut microbiota in the host defense against infection.
We constructed C57BL/6J mice with KLA and analyzed the diversity and richness of the intestinal microflora by 16S rRNA sequencing. Next, to create a microbiota-depleted (MD) mouse model, we administered multiple broad-spectrum antibiotics and validated the model using 16S rRNA sequencing. At 48 h after infection, we assessed the general health condition, liver injury, bacterial loads, and inflammatory factor levels in MD+KLA mice. Additionally, fecal microbiota transplantation (FMT) was conducted in another group of MD+KLA mice prior to infection, and we assessed whether the transplantation changed the outcomes.
The diversity of the intestinal flora was significantly changed in KLA mice compared to control mice, with a decrease in beneficial bacteria and an increase in harmful bacteria. The MD+KLA mice exhibited impaired antimicrobial capacity, reduced survival, increased inflammation and liver damage at 48 h after infection compared to the KLA mice. However, FMT normalized the inflammatory cytokine levels, reduced liver damage, and increased survival.
This study identified the gut microbiota as a protective factor against infection. The role of FMT in KLA should be investigated in future clinical studies.
我们构建了KLA的C57BL/6J小鼠，并通过16S rRNA测序分析肠道微生物群的多样性和丰富度。接下来，为了创建微生物群耗竭（MD）小鼠模型，我们给予多种广谱抗生素，并使用16S rRNA测序验证该模型。在感染后48小时，我们评估了MD+KLA小鼠的一般健康状况、肝损伤、细菌载量和炎症因子水平。此外，在另一组MD+KLA小鼠感染前进行了粪便微生物群移植（FMT），我们评估了移植是否改变了结果。
与对照小鼠相比，KLA小鼠肠道菌群的多样性发生了显著变化，有益菌减少，有害菌增加。与KLA小鼠相比，MD+KLA小鼠在感染后48小时表现出抗菌能力受损、存活率降低、炎症和肝损伤增加。然而，FMT使炎症细胞因子水平正常化，减少了肝损伤，并提高了存活率。
本研究确定肠道微生物群是抵御感染的保护因素。FMT在KLA中的作用应在未来的临床研究中进行调查。
