ElSohly Mahmoud A, Murphy Timothy P, Khan Ikhlas, Walker Larry W, Gul Waseem
ElSohly Laboratories, Inc., Oxford, Mississippi, USA.
National Center for Natural Products Research (NCNPR), School of Pharmacy, University of Mississippi, Oxford, Mississippi, USA.
Med Cannabis Cannabinoids. 2020 Aug 13;3(1):1-13. doi: 10.1159/000509550. eCollection 2020 Aug.
Hemp products are readily available and are aggressively marketed for their health and medicinal benefits. Most consumers of these products are interested because of cannabidiol (CBD), which has taken the natural products industry by storm. The CBD and Δ-tetrahydrocannabinol (Δ-THC) concentrations in these products are often absent, and even where labeled, the accuracy of the label amounts is often questionable. In order to gain a better understanding of the CBD content, fifty hemp products were analyzed by gas chromatography coupled with mass spectrometry (GC-MS) for CBD, Δ-THC, tetrahydrocannabinolic acid (Δ-THCAA), and cannabidiolic acid (CBDA). Δ-THCAA and CBDA are the natural precursors of Δ-THC and CBD in the plant material. Decarboxylation to Δ-THC and CBD is essential to get the total benefit of the neutral cannabinoids. Therefore, analysis for the neutral and acid cannabinoids is important to get a complete picture of the chemical profile of the products. The GC-MS method used for the analysis of these products was developed and validated. A 10-m × 0.18-mm DB-1 (0.4 μ film) column was used for the analysis. The majority of the hemp products were oils, one of the products was hemp butter, one was a concentrated hemp powder capsule, and another was a hemp extract capsule. Most of the products contained less than 0.1% CBD and less than 0.01% Δ-THC. Three products contained 0.1-1% CBD, and 2 products contained 0.1-0.9% Δ-THC. All of the samples appeared to be decarboxylated since the CBDA and Δ-THCAA results were less than 0.001%. The developed method is simple, sensitive, and reproducible for the detection of Δ-THC, Δ-THCAA, CBD, and CBDA in CBD oil/hemp products.
大麻产品很容易获得，并且因其健康和药用益处而被大力推销。这些产品的大多数消费者感兴趣是因为大麻二酚（CBD），它在天然产品行业掀起了风暴。这些产品中CBD和Δ-四氢大麻酚（Δ-THC）的浓度常常缺失，即使有标注，标注含量的准确性也常常存疑。为了更好地了解CBD含量，采用气相色谱-质谱联用（GC-MS）对50种大麻产品进行了分析，检测其中的CBD、Δ-THC、四氢大麻酚酸（Δ-THCAA）和大麻二酚酸（CBDA）。Δ-THCAA和CBDA是植物材料中Δ-THC和CBD的天然前体。脱羧生成Δ-THC和CBD对于充分获得中性大麻素的益处至关重要。因此，分析中性和酸性大麻素对于全面了解产品的化学概况很重要。用于分析这些产品的GC-MS方法已经开发并验证。分析采用一根10 m×0.18 mm的DB-1（0.4 μm膜厚）色谱柱。大多数大麻产品是油类，其中一种产品是大麻黄油，一种是浓缩大麻粉胶囊，另一种是大麻提取物胶囊。大多数产品的CBD含量低于0.1%，Δ-THC含量低于0.01%。三种产品的CBD含量为0.1%-1%，两种产品的Δ-THC含量为0.1%-0.9%。由于CBDA和Δ-THCAA的检测结果均小于0.001%，所有样品似乎都已脱羧。所开发的方法对于检测CBD油/大麻产品中的Δ-THC、Δ-THCAA、CBD和CBDA而言简单、灵敏且可重复。
