Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India.
Maharaja Sayajirao University of Baroda, Vadodara, India.
J Dermatol. 2021 Dec;48(12):1936-1939. doi: 10.1111/1346-8138.16165. Epub 2021 Oct 21.
We have developed a polymerase chain reaction-based method to detect and distinguish Mycobacterium leprae and Mycobacterium lepromatosis using a single set of primers based on a 45-bp difference in the amplicon size of their rpoT gene. This method can also help in detecting the cases of co-infection in a single experiment.
我们开发了一种基于聚合酶链反应的方法,使用基于 rpoT 基因扩增子大小差异 45bp 的一组引物来检测和区分麻风分枝杆菌和麻风分枝杆菌。这种方法还可以帮助在单次实验中检测合并感染的病例。