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采用新型聚合酶链反应引物同时检测和区分麻风分枝杆菌和麻风分枝杆菌。

Simultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers.

机构信息

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India.

Maharaja Sayajirao University of Baroda, Vadodara, India.

出版信息

J Dermatol. 2021 Dec;48(12):1936-1939. doi: 10.1111/1346-8138.16165. Epub 2021 Oct 21.

DOI:10.1111/1346-8138.16165
PMID:34676580
Abstract

We have developed a polymerase chain reaction-based method to detect and distinguish Mycobacterium leprae and Mycobacterium lepromatosis using a single set of primers based on a 45-bp difference in the amplicon size of their rpoT gene. This method can also help in detecting the cases of co-infection in a single experiment.

摘要

我们开发了一种基于聚合酶链反应的方法,使用基于 rpoT 基因扩增子大小差异 45bp 的一组引物来检测和区分麻风分枝杆菌和麻风分枝杆菌。这种方法还可以帮助在单次实验中检测合并感染的病例。

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