Stashenko P, Peros W J, Gibbons R J, Dearborn S M
Arch Oral Biol. 1986;31(7):455-61. doi: 10.1016/0003-9969(86)90019-1.
Five monoclonal antibodies directed against Streptococcus mutans strain JBP lipoteichoic acid (LTA) were characterized. They were all similarly reactive with the immunizing LTA-containing extract, with intact Strep. mutans JBP cells and with LTA purified from Lactobacillus casei. Immobilized anti-LTA antibodies removes LTA from LTA-containing extracts. The binding of antibodies to LTA was inhibited by the aqueous extract but not by the organic extract of de-acylated LTA, indicating reactivity with the polyglycerol-phosphate portion of the molecule. Antibodies were reactive with all serotypes of Strep. mutans, as well as with strains of Streptococcus salivarius, Streptococcus sanguis and L. casei, but not with LTA-negative species Streptococcus mitis or Actinomyces viscosus. Anti-LTA antibodies at doses of 0.3 or 3.0 micrograms/ml, had no effect on the adherence of Strep. mutans JBP to experimental salivary pellicles formed on hydroxyapatite, but enhanced adherence 150-300 per cent at 30 micrograms/ml. There was no effect of anti-LTA antibodies in a chemostat model which measured sucrose-dependent plaque accumulation by Strep. mutans. The results argue against a major role for LTA in Strep. mutans adherence or plaque accumulation in vitro.
对5种针对变形链球菌JBP菌株脂磷壁酸（LTA）的单克隆抗体进行了特性分析。它们与含免疫LTA的提取物、完整的变形链球菌JBP细胞以及从干酪乳杆菌中纯化的LTA反应相似。固定化抗LTA抗体可从含LTA的提取物中去除LTA。脱酰基LTA的水提取物可抑制抗体与LTA的结合，但有机提取物则无此作用，这表明抗体与该分子的聚甘油磷酸部分发生反应。抗体与变形链球菌的所有血清型以及唾液链球菌、血链球菌和干酪乳杆菌菌株均有反应，但与LTA阴性的缓症链球菌或黏性放线菌无反应。剂量为0.3或3.0微克/毫升的抗LTA抗体对变形链球菌JBP黏附于在羟基磷灰石上形成的实验性唾液薄膜没有影响，但在30微克/毫升时可使黏附增加150% - 300%。在一个恒化器模型中，抗LTA抗体对测量变形链球菌蔗糖依赖性菌斑积累没有影响。结果表明，LTA在体外变形链球菌黏附或菌斑积累中不起主要作用。
