Pearson C H, Pringle G A
Arch Oral Biol. 1986;31(8):541-8. doi: 10.1016/0003-9969(86)90148-2.
Proteoglycans were extracted with 4 M guanidinium chloride at 6 degrees C and purified by ion-exchange chromatography and precipitation with cetyl-pyridinium chloride. Chromatography on Sepharose CL-4B under dissociating conditions separated larger (PG1) and smaller (PG2) proteoglycans. Gingival PG2, by virtue of its amino-acid composition and the exclusive presence of L-iduronate-rich dermatan sulphate, was a proteodermatan sulphate (PDS) with a similar molecular weight to periodontal-ligament PDS. Reaction with four monoclonal antibodies to bovine skin PDS confirmed the relationship between these small proteoglycans and that of skin. Their glycoprotein cores, liberated by digestion with chondroitinase ABC, were similar in size (mol. wt = 55,000 by SDS-gel electrophoresis). Pulp PG2 had a small amount of PDS but the main component contained D-glucuronate-rich sulphated galactosaminoglycans. Similar galactosaminoglycans, which included chondroitin sulphate, characterized the larger proteoglycans of gingiva and pulp; significant amounts of L-iduronic acid-rich dermatan sulphate or heparan sulphate were not present.
蛋白聚糖在6℃下用4M氯化胍提取，通过离子交换色谱法和用十六烷基氯化吡啶沉淀进行纯化。在解离条件下于琼脂糖CL-4B上进行色谱分离，得到较大的（PG1）和较小的（PG2）蛋白聚糖。牙龈PG2由于其氨基酸组成以及富含L-艾杜糖醛酸的硫酸皮肤素的独特存在，是一种硫酸皮肤素蛋白聚糖（PDS），其分子量与牙周膜PDS相似。与四种抗牛皮肤PDS单克隆抗体的反应证实了这些小蛋白聚糖与皮肤蛋白聚糖之间的关系。经软骨素酶ABC消化释放出的它们的糖蛋白核心，大小相似（通过SDS-凝胶电泳，分子量=55,000）。牙髓PG2含有少量的PDS，但主要成分包含富含D-葡萄糖醛酸的硫酸化半乳糖胺聚糖。类似的半乳糖胺聚糖，包括硫酸软骨素，是牙龈和牙髓较大蛋白聚糖的特征；不存在大量富含L-艾杜糖醛酸的硫酸皮肤素或硫酸乙酰肝素。
