Bartold P M, Wiebkin O W, Thonard J C
Biochem J. 1983 Apr 1;211(1):119-27. doi: 10.1042/bj2110119.
Proteoglycans extracted from separated specimens of healthy human gingival epithelium and from connective tissue have been purified. The epithelial proteoglycans fractionated as a single included peak on Sepharose 4B-CL and contained heparan sulphate and dermatan sulphate glycosaminoglycans. The connective-tissue proteoglycans separated into three major populations on Sepharose 4B-CL, one of which was excluded from this gel under associative conditions (0.5 M-sodium acetate, pH 7.4). Subsequent fractionation of the excluded material under dissociative conditions (4 M-guanidinium chloride/0.05 M-sodium acetate, pH 7.4) revealed an absence of any aggregate formation of molecules within this population. The connective-tissue proteoglycans contained heparan sulphate, dermatan sulphate and chondroitin 4-sulphate, the proportions of which varied with the molecular size of the proteoglycans. Amino acid analysis of the protein cores of gingival-epithelial and connective-tissue proteoglycans revealed differences that were similar to the differences described between other types of proteoglycans such as those from skin.
已对从健康人牙龈上皮和结缔组织的分离标本中提取的蛋白聚糖进行了纯化。上皮蛋白聚糖在琼脂糖4B-CL上分离为一个单一的包含峰，含有硫酸乙酰肝素和硫酸皮肤素糖胺聚糖。结缔组织蛋白聚糖在琼脂糖4B-CL上分离为三个主要群体，其中一个在缔合条件下（0.5M醋酸钠，pH7.4）被该凝胶排除。随后在解离条件下（4M氯化胍/0.05M醋酸钠，pH7.4）对排除的物质进行分级分离，结果显示该群体内分子不存在任何聚集形成。结缔组织蛋白聚糖含有硫酸乙酰肝素、硫酸皮肤素和硫酸软骨素4-硫酸酯，其比例随蛋白聚糖的分子大小而变化。牙龈上皮和结缔组织蛋白聚糖蛋白核心的氨基酸分析显示出的差异与其他类型蛋白聚糖（如来自皮肤的蛋白聚糖）之间描述的差异相似。
